Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Multi-Layer Wear and Tool Life Calculation for Forging Applications Considering Dynamical Hardness Modeling and Nitrided Layer Degradation. [PDF]
Materials (Basel), 2020 Behrens BA +4 more
europepmc +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Heat-treated substrate facilitates interrupted cutting of PCD tools
Jin'gangshi yu moliao moju gongcheng, 2018 Polycrystalline diamond (PCD) tools have poor thermal toughness, thus less used in interrupted machining with impact. To improve the performance of PCD tools, the substrate was heat-treated to strengthen its bond with PCD and new PCD tools were made to ...
SU Yanbin, SHAO Huili, WU Ruige
doaj
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Jin'gangshi yu moliao moju gongcheng, 2017 The cutting experiments of high quality medium carbon steel 45# and GCr15 bearing sleeve are conducted using two kinds of carbide cutting inserts with different grades and different groove profile.According to the metal cutting principle and analytical ...
HE Genghuang +4 more
doaj
Investigation of cutting conditions on tool life in shoulder milling of Ti6Al4V using PVD coated micro-grain carbide insert based on design of experiments. [PDF]
Heliyon, 2020 Tatar K, Sjöberg S, Andersson N.
europepmc +1 more source
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source