Results 61 to 70 of about 431,067 (243)

ESR1 methylation and ESR1 mutations in circulating tumor cells (CTCs) and paired plasma‐cfDNA of advanced breast cancer patients: A feasibility proof‐of‐concept study

Molecular Oncology, EarlyView.
Circulating tumor cells (CTCs) and plasma cell‐free DNA (cfDNA) were analyzed to detect ESR1 mutations and methylation in patients with advanced breast cancer. CTC‐derived DNA showed higher sensitivity for mutation detection and revealed complementary genetic and epigenetic alterations, highlighting the added value of CTC analysis for understanding ...
Dimitra Stergiopoulou, Athina Markou, Eleonora Nicolo, Mara S Serafini, Qiang Zhang, Youbin Zhang, Lorenzo Gerratana, Andrew A. Davis, Huiping Liu, William J Gradishar, Carolina Reduzzi, Massimo Cristofanilli, Evi Lianidou   +12 more
wiley   +1 more source

Hydrate-based technique for natural gas processing: Experimental study of pressure-dropping and continuous modes

Chemical Engineering Journal Advances
Gas hydrate crystallization is perspective and energy-efficient technology for gas mixtures processing, including natural gas. There were compared pressure-dropping and continuous gas hydrate crystallization methods for separation of gas mixture closed ...
Anton N. Petukhov, Artem A. Atlaskin, Maria S. Kudryavtseva, Dmitry N. Shablykin, Ekaterina A. Stepanova, Ivan D. Zanozin, Dmitry M. Zarubin, Olga V. Kazarina, Maria E. Atlaskina, Artyom N. Markov, Anna N. Stepakova, Hao Lin, Anton N. Lukoyanov, Andrey V. Vorotyntsev, Gan-Ji Zhong, Ilya V. Vorotynstev   +15 more
doaj   +1 more source

Patient therapy outcome modeling in cancer organoids is improved by cancer‐associated fibroblasts and organoid assembly convolution

Molecular Oncology, EarlyView.
Patient‐derived organoids (PDOs) from pancreatic, colorectal, and gastric cancers were used to evaluate standard and experimental therapies. Incorporating cancer‐associated fibroblasts (CAFs) into organoid cultures improved patient therapy outcome prediction.
Marcin Grochowski, Liudmyla Dolinchuk, Michał Jerzak, Albert Gandurski, Tomasz Grochowski, Weronika Wojtyś, Maciej Zadrożny, Wojciech Kaźmierczak, Małgorzata Lenarcik, Marta Matejak‐Górska, Radosław Samsel, Tomasz Olesiński, Dawid Walerych   +12 more
wiley   +1 more source

Parameter Optimization of Jet Control for Compression Ramps of Two-DimensionalInlet Based on Isight

气体物理
Two-dimensional inlet is usually used in the wide-speed-range aircraft which has a higher flying velocity region. It is difficult to meet more requirements under both low-speed and high-speed flow conditions.
Fengtao SUN, Zhiwei SHI, Weilin ZHANG, Baozheng DING, Yanlin SHU   +4 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Effect of Bluff Body Attributes on Afterburner Combustion Characteristics of a Boron Solid Rocket Scramjet

International Journal of Aerospace Engineering
In order to improve the combustion efficiency of boron-containing primary gas in the secondary combustion chamber of a solid rocket scramjet and improve engine performance, the effects of different types of blunt bodies, number of rear blunt bodies, and ...
Jiang Ling, Chaoqi Xu, Yihua Xu, Haijun Sun   +3 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Numerical Study on the Aerodynamic Performance of a UAV S-Shaped Inlet with Grilles

Aerospace
This investigation designs grilles of two configurations inside an S-shaped inlet for UAVs. The present work numerically investigates the effects of the configurations, numbers, diameters, and lengths of the grilles on the inlet aerodynamic performance ...
Shu Yang, Mingshuang Shi, Dongpo Li, Zhenlong Wu, Huijun Tan, Jiahao Ren, Liming Yang   +6 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source