Results 101 to 110 of about 530,939 (289)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Automation of a Positron-emission Tomography (PET) Radiotracer Synthesis Protocol for Clinical Production. [PDF]
, 2018 The development of new positron-emission tomography (PET) tracers is enabling researchers and clinicians to image an increasingly wide array of biological targets and processes.
Collins, Jeffrey +5 more
core
Tracer diffusion inside fibrinogen layers
, 2014 We investigate the obstructed motion of tracer (test) particles in crowded environments by carrying simulations of two-dimensional Gaussian random walk in model fibrinogen monolayers of different orientational ordering.
Ewa Gudowska-Nowak +5 more
core +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Investigation of tracer diffusion in crowded cylindrical channel
, 2012 Based on a coarse-grained model, we carry out molecular dynamics simulations to analyze the diffusion of a small tracer particle inside a cylindrical channel whose inner wall is covered with randomly grafted short polymeric chains.
Chakrabarti, Rajarshi +3 more
core +2 more sources
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Patient Experience Journal, 2018 Patient Family Advisors (PFAs) are integral partners in quality improvement processes at Children’s Mercy Kansas City. Mimicking Joint Commission patient tracers, the Family Experience Tracer program was created to gather perspectives from end users of ...
Kathryn Taff +2 more
doaj
Clinical Ophthalmology, 2020 Nathaniel Tracer,1 Jaime E Dickerson Jr,2,3 Nathan M Radcliffe4 1NYU School of Medicine, New York, NY, USA; 2Sight Sciences, Inc, Menlo Park, CA, USA; 3North Texas Eye Research Institute, UNTHSC, Fort Worth, TX, USA; 4Mt Sinai School of Medicine, New ...
Tracer N, Dickerson JE Jr, Radcliffe NM
doaj
Tracer dispersion in the turbulent convective layer
, 2012 Experimental results for passive tracer dispersion in the turbulent surface layer under convective conditions are presented. In this case, the dispersion of tracer particles is determined by the interplay of two mechanisms: buoyancy and advection. In the
DuBois, Timothy C +2 more
core +1 more source