Results 91 to 100 of about 285,924 (259)

Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method

Frontiers in Neural Circuits, 2014
Here we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive TET-ON/TET-OFF system in neurons connecting two ...
Akiya eWatakabe, Akiya eWatakabe, Masafumi eTakaji, Shigeki eKato, Kazuto eKobayashi, Hiroaki eMizukami, Keiya eOzawa, Sonoko eOhsawa, Ryosuke eMatsui, Dai eWatanabe, Tetsuo eYamamori, Tetsuo eYamamori   +11 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

Quantifying the contribution of sediment sources upstream of Anzali wetland in north Iran using the fingerprinting technique

Hydrology Research
Soil erosion and sediment yield are major challenges in environmental science. Identifying sediment sources is crucial for effective watershed management and cost-efficient sediment control.
Hossein Asadi, Eisa Ebrahimi, Mohammad Rahmani, Elham Alidoust   +3 more
doaj   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Ozone loss derived from balloon-borne tracer measurements in the 1999/2000 Arctic winter [PDF]

, 2005
Balloon-borne measurements of CFC11 (from the DIRAC in situ gas chromatograph and the DESCARTES grab sampler), ClO and O3 were made during the 1999/2000 Arctic winter as part of the SOLVE-THESEO 2000 campaign, based in Kiruna (Sweden).
Ray, E., S. Dagnesjo, J. A. Pyle, A. D. Robinson, F. Danis, F. Moore, J. D. McIntyre, Deshler, Terry, Harris, N. R. P., Toohey, D. W., E. Ray, Pyle, John A., Dagnesjo, Saga, D. W. Toohey, U. Schmidt, Ray, Eric, Engel, Andreas, M. Müller, F. Goutail, A. Engel, Robinson, Alan D., Kirkwood, S., Deshler, T., J. Arvelius, Moore, Fred L., Guirlet, M., Danis, François, Nilsson, H., Dagnesjo, S., Nilsson, Hans, Elkins, J. W., Pyle, J. A., Moore, F., T. Deshler, N. R. P. Harris, Schmidt, Ulrich (Prof. Dr.), Arvelius, Johan, Schmidt, U., Engel, A., Lee, Adrian M., Millard, G. A., Toohey, Darin W., Müller, Melanie, Muller, M., S. Kirkwood, A. M. Lee, Pommereau, J. P., Goutail, F., J.-P. Pommereau, Pommereau, Jean-Pierre, Robinson, A. D., Harris, Neil R. P., M. Guirlet, H. Nilsson, Goutail, Florence, Guirlet, Marielle, McIntyre, J. D., Lee, A. M., Millard, Genevieve A., Elkins, James William, Danis, F., J. W. Elkins, Nilsson, Hampus, Arvelius, J., G. A. Millard, Kirkwood, Sheila   +65 more
core   +2 more sources

Family Experience Tracers: Patient Family Advisor led interviews generating detailed qualitative feedback to influence performance improvement

Patient Experience Journal, 2018
Patient Family Advisors (PFAs) are integral partners in quality improvement processes at Children’s Mercy Kansas City. Mimicking Joint Commission patient tracers, the Family Experience Tracer program was created to gather perspectives from end users of ...
Kathryn Taff, Sheryl Chadwick, DeeJo Miller   +2 more
doaj  

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source