Results 81 to 90 of about 1,519,271 (256)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Hospital acquired drug resistant pathogens infections in patients with viral respiratory tract infections: a retrospective study

BMC Infectious Diseases
Background Viral respiratory infections (VRTIs) caused by influenza (Flu) and COVID-19 pose significant global health challenges. Clinical outcomes are further exacerbated by infections with hospital acquired drug resistant pathogens (DRPs).
Zibo Fan, Xinmin Xu, Qun Li, Tong Zhou, Aibin Wang, Chengjie Ma, Zhihai Chen, Lianhe Lu, Yuanyuan Zhang, Yajie Wang, Wei Zhang   +10 more
doaj   +1 more source

Blind Direction-of-Arrival Estimation with Uniform Circular Array in Presence of Mutual Coupling

International Journal of Antennas and Propagation, 2016
A blind direction-of-arrival (DOA) estimation algorithm based on the estimation of signal parameters via rotational invariance techniques (ESPRIT) is proposed for a uniform circular array (UCA) when strong electromagnetic mutual coupling is present ...
Song Liu, Lisheng Yang, Shizhong Yang, Qingping Jiang, Haowei Wu   +4 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

The Assembly of Alginate–Chitosan–Xanthine Hydrogel and Halomonas Improves Soil Quality of Protected Crop Growth Under Saline–Alkali Stress

Molecules
Soil saline–alkali stress has become a great risk, leading to significant decline in crop yields, especially in protected agriculture that is frequently threatened by excessive chemical fertilization.
Rou Liu, Zirun Zhao, Guangbo Feng, Jiawen Yu, Xiaoxiang Zhou, Zijia Zhao, Danni Wang, Mingchun Li, Qilin Yu   +8 more
doaj   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

Study on the Influence of Space–Time Adaptive Processor on Single Point Position and Real‐Time Kinematic for GNSS Antenna Array Anti‐jamming Receiver

IET Radar, Sonar & Navigation
Space‐time adaptive processor (STAP) has been widely used in the GNSS antenna array anti‐jamming receiver. The cost of STAP algorithms, including the minimum variance distortion‐less response (MVDR) algorithm and power inversion (PI) algorithm, is ...
Yaoding Wang, Xiaozhou Ye, Si Chen, Zhenxin Liu   +3 more
doaj   +1 more source