Results 11 to 20 of about 30,445 (234)

microRNA-Directed Phasing during Trans-Acting siRNA Biogenesis in Plants [PDF]

Cell, 2005
Plants and animals use small RNAs (microRNAs [miRNAs] and siRNAs) as guides for posttranscriptional and epigenetic regulation. In plants, miRNAs and trans-acting (ta) siRNAs form through distinct biogenesis pathways, although they both interact with target transcripts and guide cleavage.
Edwards Allen, Zhixin Xie, Adam M Gustafson, James C. Carrington   +3 more
semanticscholar   +4 more sources

Biogenesis of <i>trans</i>-acting siRNAs, endogenous secondary siRNAs in plants

Genes & Genetic Systems, 2013
Trans-acting small interfering RNAs (tasiRNAs) are plant-specific endogenous siRNAs that control non-identical mRNAs via cleavage. The production of tasiRNAs is triggered by cleavage of capped and polyadenylated primary TAS transcripts (pri-TASs) by ...
Manabu Yoshikawa
semanticscholar   +5 more sources

Specification of Leaf Polarity in Arabidopsis via the trans-Acting siRNA Pathway [PDF]

Current Biology, 2006
Plants leaves develop proximodistal, dorsoventral (adaxial-abaxial), and mediolateral patterns following initiation. The Myb domain gene PHANTASTICA (PHAN) is required for adaxial fate in many plants , but the Arabidopsis ortholog ASYMMETRIC LEAVES1 (AS1) has milder effects, suggesting that alternate or redundant pathways exist .
Damien Garcia, Sarah A. Collier, Mary E. Byrne, Robert A. Martienssen   +3 more
semanticscholar   +5 more sources

Bioinformatic prediction and experimental validation of a microRNA-directed tandem trans-acting siRNA cascade in Arabidopsis [PDF]

Proceedings of the National Academy of Sciences, 2007
Small RNAs play pivotal roles in regulating gene expression in higher eukaryotes. Among them, trans-acting siRNAs (ta-siRNAs) are a class of small RNAs that regulate plant development. The biogenesis of ta-siRNA depends on microRNA-targeted cleavage followed by the DCL4-mediated production of small RNAs phased in 21-nt increments relative ...
Ho‐Ming Chen, Yihang Li, Shu‐Hsing Wu   +2 more
semanticscholar   +5 more sources

Artificial trans-Acting siRNAs Confer Consistent and Effective Gene Silencing [PDF]

Plant Physiology, 2008
Abstract Manipulating gene expression is critical to exploring gene function and a useful tool for altering commercial traits. Techniques such as hairpin-based RNA interference, virus-induced gene silencing, and artificial microRNAs take advantage of endogenous posttranscriptional gene silencing pathways to block translation of ...
María de la Luz Gutiérrez-Nava, Milo J. Aukerman, Hajime Sakai, Scott Tingey, Robert W. Williams   +4 more
openalex   +5 more sources

Endogenous trans-Acting siRNAs Regulate the Accumulation of Arabidopsis mRNAs [PDF]

Molecular Cell, 2004
Here we describe a set of endogenous short interfering RNAs (siRNAs) in Arabidopsis, some of which direct the cleavage of endogenous mRNAs. These siRNAs correspond to both sense and antisense strands of a noncoding RNA (At2g27400) that apparently is converted to double-stranded RNA and then processed in 21 nt increments.
Franck Vazquez, Hervé Vaucheret, Ramya Rajagopalan, Christelle Lepers, Virginie Gasciolli, Allison C. Mallory, Jean‐Louis Hilbert, David P. Bartel, Patrice Crété   +8 more
openalex   +5 more sources

DH2-dependent trans-acting siRNAs regulate leaf and lemma development in rice [PDF]

Frontiers in Plant Science
In most crops, the development of lateral organs such as leaves and floral organs plays important roles in the architecture of plants and grains and then determines the yield. Establishment of polarity in these lateral organs is one of the most critical events for their morphogenesis.
Jun Tang, Tianye Li, Yuanzhuo Gao, Xinghang Li, Ziheng Huang, Hui Zhuang, Guanghua He, Hongfa Luo, Yunfeng Li   +8 more
openalex   +4 more sources

DRB4-Dependent TAS3 trans-Acting siRNAs Control Leaf Morphology through AGO7 [PDF]

Current Biology, 2006
trans-acting siRNAs (ta-siRNAs) are endogenous RNAs that direct the cleavage of complementary mRNA targets . TAS gene transcripts are cleaved by miRNAs; the cleavage products are protected against degradation by SGS3, copied into dsRNA by RDR6, and diced into ta-siRNAs by DCL4 .
Xavier Adenot, Taline Elmayan, Dominique Lauressergues, Stéphanie Boutet, Nicolas Bouché, Virginie Gasciolli, Hervé Vaucheret   +6 more
openalex   +7 more sources

Partially Redundant Functions of Arabidopsis DICER-like Enzymes and a Role for DCL4 in Producing trans-Acting siRNAs [PDF]

Current Biology, 2005
Arabidopsis encodes four DICER-like (DCL) proteins. DCL1 produces miRNAs, DCL2 produces some virus-derived siRNAs, and DCL3 produces endogenous RDR2-dependent siRNAs, but the role of DCL4 is unknown. We show that DCL4 is the primary processor of endogenous RDR6-dependent trans-acting siRNAs (tasiRNAs).
Virginie Gasciolli, Allison C. Mallory, David P. Bartel, Hervé Vaucheret   +3 more
openalex   +5 more sources

A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis [PDF]

Genes & Development, 2005
The Arabidopsis genes, TAS2 and TAS1a, produce structurally similar noncoding transcripts that are transformed into short (21-nucleotide [nt]) and long (24-nt) siRNAs by RNA silencing pathways. Some of these short siRNAs direct the cleavage of protein-coding transcripts, and thus function as trans-acting siRNAs (ta-siRNAs).
Manabu Yoshikawa, Angela Peragine, Mee Yeon Park, R. Scott Poethig   +3 more
openalex   +4 more sources