Results 11 to 20 of about 46,086 (263)

Predicted Trans-Acting siRNAs in the Human Brain [PDF]

International Journal of Molecular Sciences, 2015
Endogenous small non-coding RNAs play pivotal roles in regulating gene expression in eukaryotes. Many studies have investigated the function and molecular mechanism of microRNAs in the development and disease of various organisms via mRNA repression of protein-coding genes. Recent findings indicate microRNAs might trigger the generation of trans-acting
Xiaoshuang Liu, Guangxin Zhang, Changqing Zhang, Jin Wang   +3 more
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Specificity of ARGONAUTE7-miR390 Interaction and Dual Functionality in TAS3 Trans-Acting siRNA Formation [PDF]

Cell, 2008
Trans-acting siRNA form through a refined RNAi mechanism in plants. miRNA-guided cleavage triggers entry of precursor transcripts into an RNA-DEPENDENT RNA POLYMERASE6 pathway, and sets the register for phased tasiRNA formation by DICER-LIKE4. Here, we show that miR390-ARGONAUTE7 complexes function in distinct cleavage or noncleavage modes at two ...
Taiowa A. Montgomery, Miya D. Howell, Josh T. Cuperus, Dawei Li, Jesse E. Hansen, Amanda L. Alexander, Elisabeth J. Chapman, Noah Fahlgren, Edwards Allen, James C. Carrington   +9 more
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Artificial trans-Acting siRNAs Confer Consistent and Effective Gene Silencing [PDF]

Plant Physiology, 2008
Abstract Manipulating gene expression is critical to exploring gene function and a useful tool for altering commercial traits. Techniques such as hairpin-based RNA interference, virus-induced gene silencing, and artificial microRNAs take advantage of endogenous posttranscriptional gene silencing pathways to block translation of ...
María de la Luz Gutiérrez-Nava, Milo J. Aukerman, Hajime Sakai, Scott Tingey, Robert W. Williams   +4 more
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Endogenous trans-Acting siRNAs Regulate the Accumulation of Arabidopsis mRNAs [PDF]

Molecular Cell, 2004
Here we describe a set of endogenous short interfering RNAs (siRNAs) in Arabidopsis, some of which direct the cleavage of endogenous mRNAs. These siRNAs correspond to both sense and antisense strands of a noncoding RNA (At2g27400) that apparently is converted to double-stranded RNA and then processed in 21 nt increments.
Franck Vazquez, Hervé Vaucheret, Ramya Rajagopalan, Christelle Lepers, Virginie Gasciolli, Allison C. Mallory, Jean‐Louis Hilbert, David P. Bartel, Patrice Crété   +8 more
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microRNA-Directed Phasing during Trans-Acting siRNA Biogenesis in Plants [PDF]

Cell, 2005
Plants and animals use small RNAs (microRNAs [miRNAs] and siRNAs) as guides for posttranscriptional and epigenetic regulation. In plants, miRNAs and trans-acting (ta) siRNAs form through distinct biogenesis pathways, although they both interact with target transcripts and guide cleavage.
Edwards Allen, Zhixin Xie, Adam M Gustafson, James C. Carrington   +3 more
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Identification of trans-acting siRNAs and their regulatory cascades in grapevine [PDF]

Bioinformatics, 2012
Abstract Motivation:  Trans-acting small interfering RNAs (ta-siRNAs) play an essential role in the regulation of plant gene expression, but relevant reports are still limited. Bioinformatic analyses indicate that many ta-siRNA-producing loci (TASs) are present in plants, implying the existence of as yet undiscovered ta-siRNAs and ...
Changqing Zhang, Guangping Li, Jin Wang, Jinggui Fang   +3 more
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DH2-dependent trans-acting siRNAs regulate leaf and lemma development in rice [PDF]

Frontiers in Plant Science
In most crops, the development of lateral organs such as leaves and floral organs plays important roles in the architecture of plants and grains and then determines the yield. Establishment of polarity in these lateral organs is one of the most critical events for their morphogenesis.
Jun Tang, Tianye Li, Yuanzhuo Gao, Xinghang Li, Ziheng Huang, Hui Zhuang, Guanghua He, Hongfa Luo, Yunfeng Li   +8 more
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DRB4-Dependent TAS3 trans-Acting siRNAs Control Leaf Morphology through AGO7 [PDF]

Current Biology, 2006
trans-acting siRNAs (ta-siRNAs) are endogenous RNAs that direct the cleavage of complementary mRNA targets . TAS gene transcripts are cleaved by miRNAs; the cleavage products are protected against degradation by SGS3, copied into dsRNA by RDR6, and diced into ta-siRNAs by DCL4 .
Xavier Adenot, Taline Elmayan, Dominique Lauressergues, Stéphanie Boutet, Nicolas Bouché, Virginie Gasciolli, Hervé Vaucheret   +6 more
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Simple gene silencing using the trans-acting siRNA pathway. [PDF]

Plant Biotechnol J, 2016
SummaryIn plants, particular micro‐RNAs (miRNAs) induce the production of a class of small interfering RNAs (siRNA) called trans‐acting siRNA (ta‐siRNA) that lead to gene silencing. A single miRNA target is sufficient for the production of ta‐siRNAs, which target can be incorporated into a vector to induce the production of siRNAs, and ultimately gene ...
Jacobs TB, Lawler NJ, LaFayette PR, Vodkin LO, Parrott WA.   +4 more
europepmc   +5 more sources

Partially Redundant Functions of Arabidopsis DICER-like Enzymes and a Role for DCL4 in Producing trans-Acting siRNAs [PDF]

Current Biology, 2005
Arabidopsis encodes four DICER-like (DCL) proteins. DCL1 produces miRNAs, DCL2 produces some virus-derived siRNAs, and DCL3 produces endogenous RDR2-dependent siRNAs, but the role of DCL4 is unknown. We show that DCL4 is the primary processor of endogenous RDR6-dependent trans-acting siRNAs (tasiRNAs).
Virginie Gasciolli, Allison C. Mallory, David P. Bartel, Hervé Vaucheret   +3 more
openalex   +5 more sources