No excessive mutations in transcription activator-like effector nuclease-mediated α-1,3-galactosyltransferase knockout Yucatan miniature pigs [PDF]
Asian-Australasian Journal of Animal Sciences, 2020 Objective Specific genomic sites can be recognized and permanently modified by genome editing. The discovery of endonucleases has advanced genome editing in pigs, attenuating xenograft rejection and cross-species disease transmission. However, off-target
Kimyung Choi +3 more
doaj +4 more sources
Generation of Fibroblasts Lacking the Sal-like 1 Gene by Using Transcription Activator-like Effector Nuclease-mediated Homologous Recombination [PDF]
Asian-Australasian Journal of Animal Sciences, 2016 The Sal-like 1 gene (Sall1) is essential for kidney development, and mutations in this gene result in abnormalities in the kidneys. Mice lacking Sall1 show agenesis or severe dysgenesis of the kidneys.
Se Eun Kim +5 more
doaj +4 more sources
Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus. [PDF]
Sci Rep, 2014 X-linked severe combined immunodeficiency (SCID-X1) caused by mutations in interleukin 2 receptor gamma (IL2RG) gene threatens the survival of affected boys during the first year of life unless hematopoietic stem cell transplantation is provided. Although viral vector-mediated gene therapy has been successfully performed in patients with no HLA-matched
Matsubara Y +6 more
europepmc +5 more sources
Transcription activator‐like effector nucleases (TALENs): An efficient tool for plant genome editing
Engineering in Life Sciences, 2016 Transcription activator‐like effector nucleases (TALENs) are a novel class of genome editing tools that provide precise insertion, deletion, or substitution of specific genes in order to alter the genome.
Kul Bhushan +2 more
doaj +3 more sources
Comparing Zinc Finger Nucleases and Transcription Activator-Like Effector Nucleases for Gene Targeting in Drosophila [PDF]
G3 Genes|Genomes|Genetics, 2013 AbstractZinc-finger nucleases have proven to be successful as reagents for targeted genome manipulation in Drosophila melanogaster and many other organisms. Their utility has been limited, however, by the significant failure rate of new designs, reflecting the complexity of DNA recognition by zinc fingers.
Kelly J. Beumer +8 more
openalex +4 more sources
Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases [PDF]
Asian-Australasian Journal of Animal Sciences, 2015 Myostatin (MSTN) is a secreted growth factor expressed in skeletal muscle and adipose tissue that negatively regulates skeletal muscle mass. Gene knockout of MSTN can result in increasing muscle mass in sheep. The objectives were to investigate whether myostatin gene can be edited in sheep by transcription activator-like effector nucleases (TALENs) in ...
Xinxia Zhao +9 more
openalex +4 more sources
High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases [PDF]
Nucleic Acids Research, 2013 Transcription activator-like effector nucleases (TALENs) are a powerful new approach for targeted gene disruption in various animal models, but little is known about their activities in Mus musculus, the widely used mammalian model organism. Here, we report that direct injection of in vitro transcribed messenger RNA of TALEN pairs into mouse zygotes ...
Zhongwei Qiu +14 more
openalex +3 more sources
Comprehensive analysis of the specificity of transcription activator-like effector nucleases [PDF]
Nucleic Acids Research, 2014 A key issue when designing and using DNA-targeting nucleases is specificity. Ideally, an optimal DNA-targeting tool has only one recognition site within a genomic sequence. In practice, however, almost all designer nucleases available today can accommodate one to several mutations within their target site.
Alexandre Juillerat +14 more
openalex +5 more sources
Correction: Corrigendum: Rh D blood group conversion using transcription activator-like effector nucleases [PDF]
Nature Communications, 2015 Nature Communications 6: Article number: 7451 (2015); Published: 16 June 2015; Updated: 22 October 2015 The original version of the Supplementary Information attached to this Article contained errors in the sequences of plasmids presented in Supplementary Note 1.
Younghoon Kim +6 more
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Rh D blood group conversion using transcription activator-like effector nucleases [PDF]
Nature Communications, 2015 AbstractGroup O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported.
Younghoon Kim +6 more
openalex +4 more sources