Cell Reports, 2014 The conserved protein Rap1 functions at telomeres in fungi, protozoa, and vertebrates. Like yeast Rap1, human Rap1 has been implicated in telomere length regulation and repression of nonhomologous end-joining (NHEJ) at telomeres. However, mouse telomeres
Shaheen Kabir +2 more
doaj +1 more source
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering [PDF]
, 2013 Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted genome modifications across a range of eukaryotes.1–7. Here we engineer this system to enable RNA-guided genome regulation in human cells by tethering transcriptional activation ...
D Bhaya +37 more
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Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering. [PDF]
PLoS ONE, 2014 Transcription activator-like (TAL) effector nucleases (TALENs) have enabled the introduction of targeted genetic alterations into a broad range of cell lines and organisms.
Jia Liu +4 more
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The transcription factors Egr2 and Egr3 are essential for the control of inflammation and antigen-induced proliferation of B and T cells [PDF]
, 2012 This article is available open access under a Creative Commons license (http://creativecommons.org/licenses/by-nc-nd/3.0/). Copyright @ 2012 Elsevier Inc.Lymphocytes provide optimal responses against pathogens with minimal inflammatory pathology. However,
Bhullar, P +7 more
core +1 more source
Newer Gene Editing Technologies toward HIV Gene Therapy
Viruses, 2013 Despite the great success of highly active antiretroviral therapy (HAART) in ameliorating the course of HIV infection, alternative therapeutic approaches are being pursued because of practical problems associated with life-long therapy.
Premlata Shankar +3 more
doaj +1 more source
The clinical potential of gene editing as a tool to engineer cell-based therapeutics
Clinical and Translational Medicine, 2020 The clinical application of ex vivo gene edited cell therapies first began a decade ago with zinc finger nuclease editing of autologous CD4+ T-cells. Editing aimed to disrupt expression of the human immunodeficiency virus co-receptor gene CCR5, with the ...
Candice Ashmore-Harris +1 more
doaj +1 more source
, 2014 Genetic engineering has always held great opportunity for the field of gene therapy. If the cause of a genetic disease can be determined, correction of this gene would allow for an efficient and permanent cure.
Harms, Carson, Lerkantitham, Serena
core +1 more source
BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]
, 2016 Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien +7 more
core +2 more sources
CAUSEL: an epigenome- and genome-editing pipeline for establishing function of noncoding GWAS variants [PDF]
, 2015 The vast majority of disease-associated single nucleotide polymorphisms (SNPs) mapped by genome-wide association studies (GWAS) are located in the non-protein coding genome, but establishing the functional and mechanistic roles of these sequence variants
Christopher Haiman +19 more
core +3 more sources
TALEN-mediated modification of the bovine genome for large-scale production of human serum albumin.
PLoS ONE, 2014 As an initial step towards creating genetically modified cattle as a biopharming source of recombinant human serum albumin (rHSA), we report modification of the bovine albumin (bA) locus by transcription activator-like effector nuclease (TALEN ...
Shaida Moghaddassi +2 more
doaj +1 more source