Results 111 to 120 of about 27,079 (225)
Organization, Transcription, and Regulation in the Animal Genome [PDF]
, 1973 This review concern recent experimental information areas of animal cell molecular biology which are relevant to the mechanism of gene regulation. New data regarding interspersion and clustering of repetitive sequence elements in DNA are considered ...
Britten, Roy J., Davidson, Eric H.
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A large-scale in vivo analysis reveals that TALENs are significantly more mutagenic than ZFNs generated using context-dependent assembly [PDF]
, 2013 Zinc-finger nucleases (ZFNs) and TAL effector nucleases (TALENs) have been shown to induce targeted mutations, but they have not been extensively tested in any animal model. Here, we describe a large-scale comparison of ZFN and TALEN mutagenicity in
Antoshechkin, Igor +7 more
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Generation and characterization of transcription activator-like effector nucleases
, 2016 Designer nucleases are developed to modify the genome in various organisms, and are promising molecular tools for the advancement of gene therapy. They should introduce only one specific double-strand or single-strand break in a complex genome in order to trigger the DNA repair mechanisms of the cell such that the DNA damage is repaired by homologous ...
Waltl, Laura +1 more
openaire +2 more sources
Methods of epigenome editing for probing the function of genomic imprinting [PDF]
, 2016 The curious patterns of imprinted gene expression draw interest from several scientific disciplines to the functional consequences of genomic imprinting.
Hill, Matthew +2 more
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Quantitative assay for TALEN activity at endogenous genomic loci
Biology Open, 2013 Summary Artificially designed nucleases such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can induce a targeted DNA double-strand break at the specific target genomic locus, leading to the frameshift ...
Yu Hisano +10 more
doaj +1 more source
A TALE of Two Nucleases: Using TALENs to Edit the Genome of C. elegans [PDF]
, 2015 Genetic engineering is an emerging technology that offers the potential to prevent, treat, or cure genetic diseases. The technology can permanently alter the genome, providing an alternative therapy to drugs and surgery.
Bartlett, Clare +2 more
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Quantifying Genome-Editing Outcomes at Endogenous Loci with SMRT Sequencing
Cell Reports, 2014 Targeted genome editing with engineered nucleases has transformed the ability to introduce precise sequence modifications at almost any site within the genome.
Ayal Hendel +7 more
doaj +1 more source
Revisiting the TALE repeat [PDF]
, 2014 Transcription activator-like (TAL) effectors specifically bind to double stranded (ds) DNA through a central domain of tandem repeats. Each TAL effector (TALE) repeat comprises 33–35 amino acids and recognizes one specific DNA base through a highly ...
Chuangye Yan +4 more
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PLoS ONE, 2018 Transcription activator-like effector nuclease (TALEN) is an artificial nuclease that causes DNA cleavage at the target site and induces few off-target reactions because of its high sequence specificity.
Hitomi Onodera +7 more
doaj +1 more source
An efficient strategy for TALEN-mediated genome engineering in Drosophila [PDF]
, 2017 In reverse genetics, a gene's function is elucidated through targeted modifications in the coding region or associated DNA cis-regulatory elements. To this purpose, recently developed customizable transcription activator-like effector nucleases (TALENs ...
Akmammedov, Arslan +5 more
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