Results 131 to 140 of about 11,860 (282)

Structure‐Guided Engineering of a Cas12i Nuclease Unlocks Near‐PAMless Genome Editing

Advanced Science, EarlyView.
CRISPR‐Cas nucleases are limited by PAM requirements, restricting genome accessibility. Structure‐guided engineering of the compact Cas12i nuclease SF01 produced three variants with near‐PAMless, enabling efficient editing at diverse 5'‐NNTN‐3' sites. These nucleases expand the editable portion of the human genome more than fourfold, enabling efficient
Qitong Chen, Hanlin Gou, Chao Xu, Sihan Wang, Huitao Zhang, Minglei Song, Mengge Wang, Xingkun Ji, Xiaofei Wei, Yuanyan Tan, Hehua Quan, Pengyu Luo, Hanyu Shou, Pengpeng Liu, Yafeng Liang, Jian‐Kang Zhu   +15 more
wiley   +1 more source

Generation of Myostatin b Knockout Yellow Catfish ( Tachysurus Fulvidraco ) Using Transcription Activator-Like Effector Nucleases

, 2014
Zhangji Dong, Jiachun Ge, Zhiqiang Xu, Xiaohua Dong, Shasha Cao, Jianlin Pan, Qingshun Zhao   +6 more
openalex   +2 more sources

Knockout of the adp gene related with colonization in B acillus nematocida   B 16 using customized transcription activator‐like effectors nucleases [PDF]

, 2015
Qiuhong Niu, Haoying Zheng, Lin Zhang, Fujun Qin, Loryn Facemire, Guo Zhang, Feng Cao, Ke‐Qin Zhang, Xiaowei Huang, Jianwei Yang, Lei He, Chanjuan Liu   +11 more
openalex   +1 more source

EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis

Advanced Science, EarlyView.
The translation factor Eukaryotic translation initiation factor 5A (eIF5A) is essential for male fertility in mice. It supports the translation of proteins crucial for heterochromatin organization and acrosome formation. eIF5A deficiency disrupts chromocenter integrity, increases chromatin accessibility, and causes transcriptional dysregulation ...
Yuling Cai, Tongtong Li, Qian Fang, Ziyou Bao, Feng Kong, Huitao Qi, Hanzhen Li, Mingyu Zhang, Wei Wang, Yongxia Guan, Wenbo Liu, Xiangfeng Chen, Zi‐Jiang Chen, Xiaohua Jiang, Xin Wang, Hongbin Liu   +15 more
wiley   +1 more source

Mechanisms of Aristolochic Acid Resistance in Specialist Butterflies and Evolutionary Insights for Potential Protective Pathways

Advanced Science, EarlyView.
The study provides an extreme example of insect adaptation to highly toxic defenses of host plants, and investigates the complex strategies to resist carcinogenic aristolochic acids, including physical isolation, metabolic detoxification, and DNA repair.
Yang Luan, Yubo Zhang, Jingjing Li, Jianqing Zhu, Yuyang Lei, Yushi Hu, Zhenqiang Xin, Tianpei Xie, Jiang Zheng, Yuanyuan Lin, Jingjing Shen, Yiyi Cao, Xinyue You, Jing Xi, Jiaying Wu, Weiying Liu, Xinyu Zhang, Yuanting Zheng, David J. Lohman, Leming Shi, Wei Zhang   +20 more
wiley   +1 more source

A One-Step System for Convenient and Flexible Assembly of Transcription Activator-Like Effector Nucleases (TALENs)

, 2016
Jinlong Zhao, Wenye Sun, Jing Liang, Jing Jiang, Zhao Wu   +4 more
openalex   +1 more source

Microgel‐Based Hierarchical Porous Hydrogel Patch with Adhesion and Resilience for Myocardial Infarction

Advanced Science, EarlyView.
This study develops a hierarchically porous hydrogel patch strategy (HPMP), based on gas‐shearing microfluidics and an aqueous two‐phase system to fabricate porous microgels as microgel‐based bioinks. The porous microgels with controllable porous structure exhibit excellent cellular behavior.
Ziyang Liu, Leyan Xuan, Yingying Hou, Ting Xie, Jieting Li, Junjie Cai, Siyu Zhang, Yingling Miao, Ning Hou, Gen He, Maobin Xie, Xiyong Yu, Mingen Xu, Guosheng Tang   +13 more
wiley   +1 more source

MAVS disruption impairs downstream signaling and results in higher virus replication levels of salmonid alphavirus subtype 3 but not infectious pancreatic necrosis virus in vitro

Frontiers in Immunology
The mitochondrial anti-viral signaling (MAVS) protein is an intermediary adaptor protein of retinoic acid-inducible gene-1 (RIG-I) like receptor (RLR) signaling, which activates the transcription factor interferon (IFN) regulatory factor 3 (IRF3) and NF ...
Cheng Xu, Amr A. A. Gamil, Xiaolin Wang, Hetron Mweemba Munang’andu, Øystein Evensen   +4 more
doaj   +1 more source

An Efficient Antiviral Strategy for Targeting Hepatitis B Virus Genome Using Transcription Activator-Like Effector Nucleases [PDF]

, 2013
Jieliang Chen, Wen Zhang, Junyu Lin, Fan Wang, Min Wu, Cuncun Chen, Ye Zheng, Xiuhua Peng, Jianhua Li, Zhenghong Yuan   +9 more
openalex   +1 more source

Transcription activator-like effector nuclease-mediated deletion safely eliminates the major egg allergen ovomucoid in chickens

, 2023
Ryo Ezaki, Tetsushi Sakuma, Daisuke Kodama, Ryou Sasahara, Taichi Shiraogawa, Kennosuke Ichikawa, Mei Matsuzaki, Akihiro Handa, Takashi Yamamoto, Hiroyuki Horiuchi   +9 more
openalex   +1 more source