High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases [PDF]
Nucleic Acids Research, 2013 Transcription activator-like effector nucleases (TALENs) are a powerful new approach for targeted gene disruption in various animal models, but little is known about their activities in Mus musculus, the widely used mammalian model organism. Here, we report that direct injection of in vitro transcribed messenger RNA of TALEN pairs into mouse zygotes ...
Zhongwei Qiu +14 more
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Transcription activator‐like effector nucleases (TALENs): An efficient tool for plant genome editing [PDF]
Engineering in Life Sciences, 2016 Transcription activator‐like effector nucleases (TALENs) are a novel class of genome editing tools that provide precise insertion, deletion, or substitution of specific genes in order to alter the genome.
Kul Bhushan +2 more
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Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering [PDF]
Plant Physiology, 2012 Abstract The ability to precisely engineer plant genomes offers much potential for advancing basic and applied plant biology. Here, we describe methods for the targeted modification of plant genomes using transcription activator-like effector nucleases (TALENs).
Yong Zhang +7 more
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TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES-AMAZING TOOL FOR GENOME EDITING [PDF]
OnLine Journal of Biological Sciences, 2013 Transcription Activator-Like Effector Nucleases (TALENs) are used to cut specific target DNA sequence in order to knock out a gene or to change its sequence. They are formed by the fusion of TALE protein serving as DNA binding domain along with non-specific DNA cleaving nucleases. Its specificity towards DNA bases in the target sequence is derived from
Singh
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Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos [PDF]
Cell & Bioscience, 2013 Abstract Transcription activator-like effector nucleases (TALENs) are novel engineered DNA nucleases, and have been proven to be effective for gene specific targeting in various species. Recently we reported gene disruptions in Xenopus embryos by using TALENs.
Yong Lei +4 more
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Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases [PDF]
Genetics, 2013 AbstractTranscription activator-like effector nucleases (TALENs) have become powerful tools for targeted genome editing. Here we demonstrate efficient targeted mutagenesis in medaka (Oryzias latipes), which serves as an excellent vertebrate model for genetics and genomics.
Satoshi Ansai +6 more
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Engineering Designer Transcription Activator‐‐Like Effector Nucleases (TALENs) by REAL or REAL‐Fast Assembly [PDF]
Current Protocols in Molecular Biology, 2012 AbstractEngineered transcription activator–like effector nucleases (TALENs) are broadly useful tools for performing targeted genome editing in a wide variety of organisms and cell types including plants, zebrafish, C. elegans, rat, human somatic cells, and human pluripotent stem cells.
Deepak Reyon +4 more
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Molecules and Cells, 2016 Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous ...
Jinlong Zhao +4 more
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Correction: Corrigendum: Rh D blood group conversion using transcription activator-like effector nucleases [PDF]
Nature Communications, 2015 Nature Communications 6: Article number: 7451 (2015); Published: 16 June 2015; Updated: 22 October 2015 The original version of the Supplementary Information attached to this Article contained errors in the sequences of plasmids presented in Supplementary Note 1.
Younghoon Kim +6 more
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Fluorescent Labeling and Visualization of Human Induced Pluripotent Stem Cells With the Use of Transcription Activator–like Effector Nucleases [PDF]
Transplantation Proceedings, 2014 The visualization of induced pluripotent stem (iPS) cells with the use of fluorescent techniques is useful for the in vivo evaluation of iPS-derived functional cells following differentiation and distribution of the transplanted cells. The data obtained from the fluorescently labeled iPS cells would lead to amelioration and validation of protocols ...
Keisuke Sekine +2 more
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