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The application of transcription activator-like effector nucleases for genome editing in C. elegans
Methods, 2014The nematode Caenorhabditis elegans has been a powerful model system for biomedical research in the past decades, however, the efficient genetic tools are still demanding for gene knockout, knock-in or conditional gene mutations. Transcription activator-like effector nucleases (TALENs) that comprise a sequence-specific DNA-binding domain fused to a ...
Guangshuo Ou, Peishan Yi, Wei Li
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Polyethylenimine-Mediated CCR5 Gene Knockout Using Transcription Activator-Like Effector Nucleases
Journal of Biomedical Nanotechnology, 2018CCR5 acts as one of the key coreceptors for human immunodeficiency virus infection, which leads to acquired immune deficiency syndrome. CCR5 gene knockout comes up as an alternative method for treatment of the disease. Transcription activator-like effector nuclease is a powerful gene editing tool characterized by the ease of design, high rates of ...
Lijun Wang +4 more
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2015
Interest is increasing in transcription activator-like effector nucleases (TALENs) as a tool to introduce targeted double-strand breaks into the large genomes of human and animal cell lines. The produced DNA lesions stimulate DNA repair pathways, error-prone but dominant non-homologous end joining (NHEJ) and accurate but less occurring homology ...
Huseyin Sumer +3 more
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Interest is increasing in transcription activator-like effector nucleases (TALENs) as a tool to introduce targeted double-strand breaks into the large genomes of human and animal cell lines. The produced DNA lesions stimulate DNA repair pathways, error-prone but dominant non-homologous end joining (NHEJ) and accurate but less occurring homology ...
Huseyin Sumer +3 more
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Biotechnology and Bioengineering, 2013
AbstractTranscription activator‐like effector (TALE) nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. The site‐specific chromosomal double‐strand breaks introduced by TALENs significantly increase the efficiency of genomic modification.
Huimin Zhao, Ning Sun
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AbstractTranscription activator‐like effector (TALE) nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. The site‐specific chromosomal double‐strand breaks introduced by TALENs significantly increase the efficiency of genomic modification.
Huimin Zhao, Ning Sun
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Editing of the heavy chain gene of Bombyx mori using transcription activator like effector nucleases
Biochemical and Biophysical Research Communications, 2014The silk gland of Bombyx mori represents an established in vivo system for producing recombinant proteins. However, low yields of recombinant proteins have limited the system's further development because endogenous silk proteins were present. Transcription activator-like effector nucleases (TALENs) tool which work in pairs to bind and cleave DNA at ...
Masao Nakagaki, Yujun Wang
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2018
Targeted genome engineering technologies are revolutionizing the field of functional genomics and have been extensively used in a variety of model organisms, including X. tropicalis and X. laevis. The original methods based on Zn-finger proteins coupled to endonuclease domains were initially replaced by the more efficient and straightforward ...
Kris Vleminckx, Tom Van Nieuwenhuysen
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Targeted genome engineering technologies are revolutionizing the field of functional genomics and have been extensively used in a variety of model organisms, including X. tropicalis and X. laevis. The original methods based on Zn-finger proteins coupled to endonuclease domains were initially replaced by the more efficient and straightforward ...
Kris Vleminckx, Tom Van Nieuwenhuysen
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2014
Targetable nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), induce DNA double-strand breaks (DSBs) into user-defined sites. DSBs are immediately repaired through the evolutionarily conserved pathways
Takashi Yamamoto, Hiroshi Ochiai
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Targetable nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), induce DNA double-strand breaks (DSBs) into user-defined sites. DSBs are immediately repaired through the evolutionarily conserved pathways
Takashi Yamamoto, Hiroshi Ochiai
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Transcription Activator-Like Effectors (TALEs) Hybrid Nucleases for Genome Engineering Application
2011Gene targeting is a powerful genome engineering tool that can be used for a variety of biotechnological applications. Genomic double-strand DNA breaks generated by engineered site-specific nucleases can stimulate gene targeting. Hybrid nucleases are composed of DNA binding module and DNA cleavage module.
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