Results 251 to 260 of about 11,860 (282)

The application of transcription activator-like effector nucleases for genome editing in C. elegans

Methods, 2014
The nematode Caenorhabditis elegans has been a powerful model system for biomedical research in the past decades, however, the efficient genetic tools are still demanding for gene knockout, knock-in or conditional gene mutations. Transcription activator-like effector nucleases (TALENs) that comprise a sequence-specific DNA-binding domain fused to a ...
Peishan, Yi, Wei, Li, Guangshuo, Ou
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Transcription Activator Like Effector Nucleases (TALENs) Mediated CD20 Gene Knockout Burkitt Lymphoma Model

Blood, 2013
Abstract Background Burkitt lymphoma (BL) is an aggressive mature B-cell malignancy and is the most common non-Hodgkin lymphoma (NHL) in children and adolescents (Cairo et al., Blood, 2007; Miles/Cairo et al., BJHaem, 2012) and the survival of pediatric Burkitt lymphoma (PBL) has significantly ...
Sanghoon Lee, Changhong Yin, Janet Ayello, Michael Genualdi, Carmella van de Ven, Mitchell S. Cairo   +5 more
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Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology

2018
Targeted genome engineering technologies are revolutionizing the field of functional genomics and have been extensively used in a variety of model organisms, including X. tropicalis and X. laevis. The original methods based on Zn-finger proteins coupled to endonuclease domains were initially replaced by the more efficient and straightforward ...
Tom, Van Nieuwenhuysen, Kris, Vleminckx
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Transcription activator‐like effector nucleases (TALENs): A highly efficient and versatile tool for genome editing

Biotechnology and Bioengineering, 2013
AbstractTranscription activator‐like effector (TALE) nucleases (TALENs) have recently emerged as a revolutionary genome editing tool in many different organisms and cell types. The site‐specific chromosomal double‐strand breaks introduced by TALENs significantly increase the efficiency of genomic modification.
Ning, Sun, Huimin, Zhao
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Editing of the heavy chain gene of Bombyx mori using transcription activator like effector nucleases

Biochemical and Biophysical Research Communications, 2014
The silk gland of Bombyx mori represents an established in vivo system for producing recombinant proteins. However, low yields of recombinant proteins have limited the system's further development because endogenous silk proteins were present. Transcription activator-like effector nucleases (TALENs) tool which work in pairs to bind and cleave DNA at ...
Yujun, Wang, Masao, Nakagaki
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328 VERSATILE TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASE (TALEN)-MEDIATED ENGINEERING OF OSSABAW MINIATURE SWINE

Reproduction, Fertility and Development, 2012
Transcription activator-like effector nucleases (TALEN) are programmable nucleases that join the modular DNA-binding domain of transcription activator-like (TAL) effectors with FokI endonuclease. TALEN-induced double-strand breaks can be used for gene inactivation via repair by nonhomologous end joining (NHEJ) or to stimulate homologous recombination ...
D. F. Carlson, W. Tan, S. C. Fahrenkrug
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Transcription Activator-Like Effectors (TALEs) Hybrid Nucleases for Genome Engineering Application

2011
Gene targeting is a powerful genome engineering tool that can be used for a variety of biotechnological applications. Genomic double-strand DNA breaks generated by engineered site-specific nucleases can stimulate gene targeting. Hybrid nucleases are composed of DNA binding module and DNA cleavage module.
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Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs).

Journal of visualized experiments : JoVE, 2017
Identifying alleles of genes underlying evolutionary change is essential to understanding how and why evolution occurs. Towards this end, much recent work has focused on identifying candidate genes for the evolution of traits in a variety of species. However, until recently it has been challenging to functionally validate interesting candidate genes ...
Johanna E, Kowalko, Li, Ma, William R, Jeffery   +2 more
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TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES (TALEN)

2021
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TALENs (Transcription Activator-Like Effector Nucleases). Dr. Tarek Kapiel

2021
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