Gene Editing of the Follicle-Stimulating Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Transcription Activator-like Effector Nuclease Technology with Electroporation. [PDF]
Biology (Basel), 2023 Qin G +8 more
europepmc +3 more sources
Efficient genome engineering by targeted homologous recombination in mouse embryos using transcription activator-like effector nucleases [PDF]
Nature Communications, 2014 Generation of mouse models by introducing transgenes using homologous recombination is critical for understanding fundamental biology and pathology of human diseases. Here we investigate whether artificial transcription activator-like effector nucleases (TALENs)-powerful tools that induce DNA double-strand breaks at specific genomic locations-can be ...
Daniel Sommer +11 more
openalex +4 more sources
Targeted Mutagenesis of the Tomato PROCERA Gene Using Transcription Activator-Like Effector Nucleases [PDF]
Plant Physiology, 2014 Transcription activator-like effector nucleases successfully generate a heritable tomato mutant.
Vai S. Lor +4 more
openalex +4 more sources
PLoS ONE, 2012 Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in zebrafish. These designer nucleases bind to and cleave DNA at particular target sites, inducing error-prone repair that can result in ...
Finola E. Moore +8 more
openalex +7 more sources
Transcription activator-like effector nuclease (TALEN) as a promising diagnostic approach for COVID-19. [PDF]
Expert Rev Mol Diagn, 2022 Behboudi E +4 more
europepmc +3 more sources
Inactivation of Hepatitis B Virus Replication in Cultured Cells and In Vivo with Engineered Transcription Activator-Like Effector Nucleases [PDF]
Molecular Therapy, 2013 Chronic hepatitis B virus (HBV) infection remains an important global health problem. Stability of the episomal covalently closed circular HBV DNA (cccDNA) is largely responsible for the modest curative efficacy of available therapy. Since licensed anti-HBV drugs have a post-transcriptional mechanism of action, disabling cccDNA is potentially of ...
Kristie Bloom +4 more
openalex +4 more sources
Simultaneous Gene Editing by Injection of mRNAs Encoding Transcription Activator-Like Effector Nucleases into Mouse Zygotes [PDF]
Molecular and Cellular Biology, 2014 Injection of transcription activator-like effector nucleases (TALEN) mRNAs into mouse zygotes transferred into foster mothers efficiently generated founder mice with heritable mutations in targeted genes. Immunofluorescence visualization of phosphorylated histone 2A (γH2AX) combined with fluorescence in situ hybridization revealed that TALEN pairs ...
Chun‐Liang Li +10 more
openalex +4 more sources
Generation of obese rat model by transcription activator-like effector nucleases targeting the leptin receptor gene [PDF]
Science China Life Sciences, 2016 Abstract The laboratory rat is a valuable mammalian model organism for basic research and drug discovery. Here we demonstrate an efficient methodology by applying transcription activator-like effector nucleases (TALENs) technology to generate Leptin receptor (Lepr) knockout rats on the Sprague Dawley (SD) genetic background.
Yuting Chen +10 more
openalex +4 more sources
PLOS ONE, 2017 Phosphatase and tensin homolog (PTEN) is a lipid and protein phosphatase that antagonizes signaling by the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway. The PTEN gene is a major tumor suppressor, with mutations of this gene occurring frequently in tumors of humans and mice.
Yuriko Matsuzaki +3 more
openalex +6 more sources
Transcription activator-like effector nuclease (TALEN)-mediated gene correction in integration-free β-thalassemia induced pluripotent stem cells. [PDF]
J Biol Chem, 2013 Ma N +11 more
europepmc +2 more sources