Results 31 to 40 of about 27,079 (225)

Production of α1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology. [PDF]

J Vet Sci, 2016
Kang JT, Kwon DK, Park AR, Lee EJ, Yun YJ, Ji DY, Lee K, Park KW.   +7 more
europepmc   +2 more sources

Transcription activator-like effector nuclease (TALEN)-mediated female-specific sterility in the silkworm, Bombyx mori. [PDF]

Insect Mol Biol, 2014
Xu J, Wang Y, Li Z, Ling L, Zeng B, James AA, Tan A, Huang Y.   +7 more
europepmc   +3 more sources

Review of knockout technology approaches in bacterial drug resistance research [PDF]

PeerJ, 2023
Gene knockout is a widely used method in biology for investigating gene function. Several technologies are available for gene knockout, including zinc-finger nuclease technology (ZFN), suicide plasmid vector systems, transcription activator-like effector
Chunyu Tong, Yimin Liang, Zhelin Zhang, Sen Wang, Xiaohui Zheng, Qi Liu, Bocui Song   +6 more
doaj   +2 more sources

Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease. [PDF]

Curr Protoc Stem Cell Biol, 2014
Luo Y, Rao M, Zou J.
europepmc   +3 more sources

Programmable Molecular Scissors: Applications of a New Tool for Genome Editing in Biotech [PDF]

Molecular Therapy: Nucleic Acids, 2019
Targeted genome editing is an advanced technique that enables precise modification of the nucleic acid sequences in a genome. Genome editing is typically performed using tools, such as molecular scissors, to cut a defined location in a specific gene ...
Subbroto Kumar Saha, Forhad Karim Saikot, Md. Shahedur Rahman, Mohammad Abu Hena Mostofa Jamal, S.M. Khaledur Rahman, S.M. Riazul Islam, Ki-Hyun Kim   +6 more
doaj   +3 more sources

Comprehensive analysis of the specificity of transcription activator-like effector nucleases [PDF]

Nucleic Acids Research, 2014
A key issue when designing and using DNA-targeting nucleases is specificity. Ideally, an optimal DNA-targeting tool has only one recognition site within a genomic sequence. In practice, however, almost all designer nucleases available today can accommodate one to several mutations within their target site.
Juillerat, Alexandre, Dubois, Gwendoline, Valton, Julien, Thomas, Séverine, Stella, Stefano, Maréchal, Alan, Langevin, Stéphanie, Benomari, Nassima, Bertonati, Claudia, Silva, George H, Daboussi, Fayza, Epinat, Jean-Charles, Montoya, Guillermo, Duclert, Aymeric, Duchateau, Philippe   +14 more
openaire   +5 more sources

Improvements of nuclease and nickase gene modification techniques for the treatment of genetic diseases

Frontiers in Genome Editing, 2022
Advancements in genome editing make possible to exploit the functions of enzymes for efficient DNA modifications with tremendous potential to treat human genetic diseases.
Yaoyao Lu, Yaoyao Lu, Cedric Happi Mbakam, Cedric Happi Mbakam, Bo Song, Bo Song, Eli Bendavid, Eli Bendavid, Jacques-P. Tremblay, Jacques-P. Tremblay   +9 more
doaj   +1 more source

Rapid assembly of customized TALENs into multiple [PDF]

, 2013
Transcriptional activator-like effector nucleases (TALENs) have become a powerful tool for genome editing. Here we present an efficient TALEN assembly approach in which TALENs are assembled by direct Golden Gate ligation into Gateway® Entry vectors from ...
AJ Bogdanove, AJ Walhout, B Davies, BP Zambrowicz, C Engler, C Mussolino, C Uhde-Stone, Christina Chan, CJ Friddle, D Reyon, DA Stroud, DF Carlson, DL Cheo, E Weber, EL Doyle, F Zhang, FE Moore, GK Taylor, H Wang, H Wang, J Boch, J Liu, JC Miller, JL Hartley, JL Schmid-Burgk, K Jacoby, Kyle E. Orwig, L Cong, L Cong, L Tesson, M Holkers, M Jinek, M Li, M Rostovskaya, M Rostovskaya, MA Li, MA Li, MJ Moscou, ML Christian, N Sun, N Sun, NE Sanjana, P Mali, Q Ding, R Alba, R Geissler, R Hu, R Li, R Takeuchi, S Ansai, S Baxter, S Chen, S Cristea, Siliang Zhang, SM Choi, T Cathomen, T Cermak, T Gaj, T Li, T Li, T Li, T Mashimo, T Sakuma, VM Bedell, Xin Huang, Y Fu, Y Kim, Y Zhang, Y Zu, YH Sung, YH Sung, Yi Sheng, Z Qiu, Z Zhang, Zhengxing Zhang   +74 more
core   +11 more sources

A Targeted In-Fusion Expression System for Recombinant Protein Production in Bombyx mori

Frontiers in Genetics, 2022
The domesticated silkworm, Bombyx mori, is an economically important insect that synthesizes large amounts of silk proteins in its silk gland to make cocoons.
Zhiqian Li, Zhiqian Li, Zhiqian Li, Lang You, Lang You, Qichao Zhang, Qichao Zhang, Ye Yu, Ye Yu, Anjiang Tan, Anjiang Tan   +10 more
doaj   +1 more source

Exploring the transcription activator-like effectors scaffold versatility to expand the toolbox of designer nucleases [PDF]

BMC Molecular Biology, 2014
The past decade has seen the emergence of several molecular tools that render possible modification of cellular functions through accurate and easy addition, removal, or exchange of genomic DNA sequences. Among these technologies, transcription activator-like effectors (TALE) has turned out to be one of the most versatile and incredibly robust platform
George H. Silva, Jérome Mikolajczak, Aymeric Duclert, Mikhail Zaslavskiy, Séverine Thomas, Alexandre Juillerat, Fayza Daboussi, Fabian Bietz, Philippe Duchateau, Marine Beurdeley, Julien Valton, Gwendoline Dubois   +11 more
openaire   +4 more sources