Frontiers in Genome Editing, 2022 Advancements in genome editing make possible to exploit the functions of enzymes for efficient DNA modifications with tremendous potential to treat human genetic diseases.
Yaoyao Lu +9 more
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A Targeted In-Fusion Expression System for Recombinant Protein Production in Bombyx mori
Frontiers in Genetics, 2022 The domesticated silkworm, Bombyx mori, is an economically important insect that synthesizes large amounts of silk proteins in its silk gland to make cocoons.
Zhiqian Li +10 more
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Strategies for mitochondrial gene editing
Computational and Structural Biotechnology Journal, 2021 Mitochondria, as the energy factory of cells, participate in metabolism processes and play a critical role in the maintenance of human life activities. Mitochondria belong to semi-automatic organelles, which have their own genome different from nuclear ...
Xingbo Yang +4 more
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Virus-Like Particle Mediated CRISPR/Cas9 Delivery for Efficient and Safe Genome Editing
Life, 2020 The discovery of designer nucleases has made genome editing much more efficient than before. The designer nucleases have been widely used for mechanistic studies, animal model generation and gene therapy development.
Pin Lyu, Luxi Wang, Baisong Lu
doaj +1 more source
Comparison of TALEN scaffolds in Xenopus tropicalis
Biology Open, 2013 Summary Transcription activator-like effector nucleases (TALENs) are facile and potent tools used to modify a gene of interest for targeted gene knockout.
Keisuke Nakajima, Yoshio Yaoita
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Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants [PDF]
Frontiers in Plant Science, 2016 Targeted DNA integration into known locations in the genome has potential advantages over the random insertional events typically achieved using conventional means of genetic modification. Specifically integrated transgenes are guaranteed to co-segregate, and expression level is more predictable, which makes downstream characterization and line ...
Forsyth, Adrienne +3 more
openaire +3 more sources
Circadian Clock Genes Regulate Temperature-Dependent Diapause Induction in Silkworm Bombyx mori
Frontiers in Physiology, 2022 The bivoltine strain of the domestic silkworm, Bombyx mori, exhibits a facultative diapause phenotype that is determined by maternal environmental conditions during embryonic and larval development.
Satoshi Homma +9 more
doaj +1 more source
Quantification of designer nuclease induced mutation rates: a direct comparison of different methods
Molecular Therapy: Methods & Clinical Development, 2016 Designer nucleases are broadly applied to induce site-specific DNA double-strand breaks (DSB) in genomic DNA. These are repaired by nonhomologous end joining leading to insertions or deletions (in/dels) at the respective DNA-locus.
Eric Ehrke-Schulz +6 more
doaj +1 more source
Transcription activator–like effector nuclease (TALEN)-mediated genome editing to generate genetically modified monkeys [PDF]
Science-Business eXchange, 2014 openalex +3 more sources
Gene targeting in rats using transcription activator-like effector nucleases
Methods, 2014 The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat.
Ménoret, Séverine +10 more
openaire +3 more sources