CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]
, 2020 The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I +2 more
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Virus-Like Particle Mediated CRISPR/Cas9 Delivery for Efficient and Safe Genome Editing
Life, 2020 The discovery of designer nucleases has made genome editing much more efficient than before. The designer nucleases have been widely used for mechanistic studies, animal model generation and gene therapy development.
Pin Lyu, Luxi Wang, Baisong Lu
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Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases [PDF]
, 2012 Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically relatively basal and comprise many pests. However, the absence of a sophisticated genetic model system, or targeted gene-manipulation system, has limited research on ...
Bando, Tetsuya +10 more
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Comparison of TALEN scaffolds in Xenopus tropicalis
Biology Open, 2013 Summary Transcription activator-like effector nucleases (TALENs) are facile and potent tools used to modify a gene of interest for targeted gene knockout.
Keisuke Nakajima, Yoshio Yaoita
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Generation of knockout rabbits using transcription activator-like effector nucleases
Cell Regeneration, 2014 Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They are composed of a non-specific cleavage domain and a tailor made DNA-binding module, which enables a broad range of genetic modifications by inducing efficient DNA double ...
Fenggong Cui +13 more
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Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
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Circadian Clock Genes Regulate Temperature-Dependent Diapause Induction in Silkworm Bombyx mori
Frontiers in Physiology, 2022 The bivoltine strain of the domestic silkworm, Bombyx mori, exhibits a facultative diapause phenotype that is determined by maternal environmental conditions during embryonic and larval development.
Satoshi Homma +9 more
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Gene targeting in rats using transcription activator-like effector nucleases
Methods, 2014 The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat.
Ménoret, Séverine +10 more
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Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. [PDF]
, 2013 Insertional therapies have shown great potential for combating genetic disease and safer methods would undoubtedly broaden the variety of possible illness that can be treated. A major challenge that remains is reducing the risk of insertional mutagenesis
Bhakta, Mital S +6 more
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Quantification of designer nuclease induced mutation rates: a direct comparison of different methods
Molecular Therapy: Methods & Clinical Development, 2016 Designer nucleases are broadly applied to induce site-specific DNA double-strand breaks (DSB) in genomic DNA. These are repaired by nonhomologous end joining leading to insertions or deletions (in/dels) at the respective DNA-locus.
Eric Ehrke-Schulz +6 more
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