Results 41 to 50 of about 11,860 (282)
Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases
eLife, 2014 Future therapeutic use of engineered site-directed nucleases, like zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), relies on safe and effective means of delivering nucleases to cells.
Yujia Cai +2 more
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Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases [PDF]
Genetics, 2013 AbstractTranscription activator-like effector nucleases (TALENs) have become powerful tools for targeted genome editing. Here we demonstrate efficient targeted mutagenesis in medaka (Oryzias latipes), which serves as an excellent vertebrate model for genetics and genomics.
Satoshi, Ansai +6 more
openaire +2 more sources
Genome Engineering of Crops with Designer Nucleases
The Plant Genome, 2012 Recent advances in the field of genome engineering indicate that it will soon be routine to make site-directed modifications to the genomes of crop species, including targeted mutations, gene insertions, and gene replacements. This new technology will be
Shaun J. Curtin +2 more
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Treatment of infectious diseases by in vivo gene editing
National Science Open, 2023 Gene editing is the specific modification of genome sequences at desired sites using technologies derived from zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic ...
Wu Yuxuan, Zhang Haokun
doaj +1 more source
Generation and characterization of transcription activator-like effector nucleases
, 2016 Designer nucleases are developed to modify the genome in various organisms, and are promising molecular tools for the advancement of gene therapy. They should introduce only one specific double-strand or single-strand break in a complex genome in order to trigger the DNA repair mechanisms of the cell such that the DNA damage is repaired by homologous ...
Waltl, Laura +1 more
openaire +2 more sources
Molecular Therapy: Nucleic Acids, 2019 The traditional healthcare system is at the doorstep for entering into the arena of molecular medicine. The enormous knowledge and ongoing research have now been able to demonstrate methodologies that can alter DNA coding.
Sikandar Hayat Khan
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RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency
Nature Communications, 2016 CRISPR/Cas9 and transcription activator-like effector nuclease (TALEN) are becoming major tools for genome editing. Here, Song et al. show that RS-1, a small-molecule enhancer for homology directed repair, increases the CRISPR/Cas9 and TALEN mediated ...
Jun Song +5 more
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Plants, 2022 At least two sets of RNA polymerase (RNAP), nucleus (NEP)- and plastid (PEP)-encoded polymerases, recognizing distinct promoters exist in the plastids of land plants.
Yu-Chang Liu +2 more
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Brazilian Journal of Medical and Biological Research, 2019 Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically.
A.S. Volobueva +2 more
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Delivering the second revolution in site-specific nucleases
eLife, 2014 Viruses have been used to deliver two types of site-specific nucleases into cells for targeted gene editing.
Perry B Hackett, Nikunj V Somia
doaj +1 more source