Results 51 to 60 of about 27,079 (225)
Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases
eLife, 2014 Future therapeutic use of engineered site-directed nucleases, like zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), relies on safe and effective means of delivering nucleases to cells.
Yujia Cai +2 more
doaj +1 more source
DevA, a GntR-like transcriptional regulator required for development in streptomyces coelicolor [PDF]
, 2006 The gram-positive filamentous bacterium Streptomyces coelicolor has a complex developmental cycle with three distinct phases: growth of the substrate mycelium, development of reproductive structures called aerial hyphae, and differentiation of these ...
Buttner, Mark J. +4 more
core +2 more sources
Genome Engineering of Crops with Designer Nucleases
The Plant Genome, 2012 Recent advances in the field of genome engineering indicate that it will soon be routine to make site-directed modifications to the genomes of crop species, including targeted mutations, gene insertions, and gene replacements. This new technology will be
Shaun J. Curtin +2 more
doaj +1 more source
Treatment of infectious diseases by in vivo gene editing
National Science Open, 2023 Gene editing is the specific modification of genome sequences at desired sites using technologies derived from zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic ...
Wu Yuxuan, Zhang Haokun
doaj +1 more source
Genome engineering of isogenic human ES cells to model autism disorders. [PDF]
, 2014 Isogenic pluripotent stem cells are critical tools for studying human neurological diseases by allowing one to study the effects of a mutation in a fixed genetic background.
Geschwind, Daniel H +10 more
core +1 more source
The bldC developmental locus of Streptomyces coelicolor encodes a member of a family of small DNA-binding proteins related to the DNA-binding domains of the MerR family. [PDF]
, 2005 The bldC locus, required for formation of aerial hyphae in Streptomyces coelicolor, was localized by map-based cloning to the overlap between cosmids D17 and D25 of a minimal ordered library. Subcloning and sequencing showed that bldC encodes a member of
Buttner, Mark J. +3 more
core +2 more sources
RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency
Nature Communications, 2016 CRISPR/Cas9 and transcription activator-like effector nuclease (TALEN) are becoming major tools for genome editing. Here, Song et al. show that RS-1, a small-molecule enhancer for homology directed repair, increases the CRISPR/Cas9 and TALEN mediated ...
Jun Song +5 more
doaj +1 more source
Plants, 2022 At least two sets of RNA polymerase (RNAP), nucleus (NEP)- and plastid (PEP)-encoded polymerases, recognizing distinct promoters exist in the plastids of land plants.
Yu-Chang Liu +2 more
doaj +1 more source
TALEN-mediated editing of the mouse Y chromosome [PDF]
, 2013 The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to ...
A Mayer +26 more
core +1 more source
Brazilian Journal of Medical and Biological Research, 2019 Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically.
A.S. Volobueva +2 more
doaj +1 more source