Results 61 to 70 of about 27,079 (225)
Highly Efficient Generation of Heritable Zebrafish Gene Mutations Using Homo- and Heterodimeric TALENs [PDF]
, 2013 Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable targeted gene disruption in a wide variety of model organisms.
Cade, Lindsay +9 more
core +1 more source
Delivering the second revolution in site-specific nucleases
eLife, 2014 Viruses have been used to deliver two types of site-specific nucleases into cells for targeted gene editing.
Perry B Hackett, Nikunj V Somia
doaj +1 more source
JOR Spine, 2023 There have been an increasing number of patients with degenerative disc diseases due to the aging population. In light of this, studies on the pathogenesis of intervertebral disc degeneration have become a hot topic, and gene knockout mice have become a ...
Ze‐Yu Lu +6 more
doaj +1 more source
dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression. [PDF]
, 2017 Distinct epigenomic profiles of histone marks have been associated with gene expression, but questions regarding the causal relationship remain. Here we investigated the activity of a broad collection of genomically targeted epigenetic regulators that ...
Farnham, Peggy J +8 more
core +2 more sources
Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources
Molecular Therapy: Nucleic Acids, 2014 Although noncancerous immortalized cell lines have been developed by introducing genes into human and murine somatic cells, such cell lines have not been available in large domesticated animals like pigs.
JoonHo Moon +6 more
doaj +1 more source
Transcription Activator-Like Effector (TALE) Nucleases and Repressor TALEs for Antiviral Gene Therapy [PDF]
Current Stem Cell Reports, 2015 Genome modification platforms are fast becoming valuable tools for the development of novel therapies. The use of sequence-specific DNA binding proteins in conjunction with various effector domains enables targeted gene editing to be employed as a mode of therapy.
Kristie Bloom +2 more
openaire +2 more sources
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation [PDF]
, 2015 The relative ease, speed and biological scope of CRISPR/Cas9-based reagents for genomic manipulations are revolutionizing virtually all areas of molecular biosciences, including functional genomics, genetics, applied biomedical research and agricultural ...
Baltes, Nicholas J. +10 more
core +1 more source
A Current Approach in Animal Breeding: CRISPR/Cas9 Genome Modification System
Turkish Journal of Agriculture: Food Science and Technology, 2016 Genome modifications include potential about providing significant advantages on increasing yield performance and developing resistance to diseases. Gene editing methods that provides silencing or expressing of a gene which is an individual already has ...
Fatih Bilgi +2 more
doaj +1 more source
Immunodeficient Rabbit Models: History, Current Status and Future Perspectives
Applied Sciences, 2020 Production of immunodeficient (ID) models in non-murine animal species had been extremely challenging until the advent of gene-editing tools: first zinc finger nuclease (ZFN), then transcription activator-like effector nuclease (TALEN), and most recently
Jun Song +7 more
doaj +1 more source