Results 71 to 80 of about 11,860 (282)
Transcription Activator-Like Effector (TALE) Nucleases and Repressor TALEs for Antiviral Gene Therapy [PDF]
Current Stem Cell Reports, 2015 Genome modification platforms are fast becoming valuable tools for the development of novel therapies. The use of sequence-specific DNA binding proteins in conjunction with various effector domains enables targeted gene editing to be employed as a mode of therapy.
Kristie Bloom +2 more
openaire +1 more source
FEBS Open Bio, EarlyView.Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold +11 more
wiley +1 more source
Advanced Functional Materials, EarlyView.Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley +12 more
wiley +1 more source
Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases [PDF]
Asian-Australasian Journal of Animal Sciences, 2016 To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases.TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair.
Yu-Guo Yuan +8 more
openaire +3 more sources
Advanced Functional Materials, EarlyView.FeDSNP‐Pa, a metallized nanoparticle loaded with sodium pyruvate (Pa), exerts triple therapeutic effects by scavenging reactive oxygen species (ROS), suppressing inflammatory responses, and inhibiting pyroptosis signaling pathways. This multifunctional neuroprotective strategy protecting retinal ganglion cells (RGCs) from elevated intraocular pressure ...
Yukun Wu +5 more
wiley +1 more source
Targeting Human α-Lactalbumin Gene Insertion into the Goat β-Lactoglobulin Locus by TALEN-Mediated Homologous Recombination. [PDF]
PLoS ONE, 2016 Special value of goat milk in human nutrition and well being is associated with medical problems of food allergies which are caused by milk proteins such as β-lactoglobulin (BLG).
Hongmei Zhu +8 more
doaj +1 more source
Advanced Functional Materials, EarlyView.This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti +3 more
wiley +1 more source
Advanced Functional Materials, EarlyView.We introduce a nucleic acid nanoparticle (NANP) platform designed to be rrecognized by the human innate immune system in a regulated manner. By changing chemical composition while maintaining constant architectural parameters, we identify key determinants of immunorecognition enabling the rational design of NANPs with tunable immune activation profiles
Martin Panigaj +21 more
wiley +1 more source
Recent advances and perspectives in crop biofortification
Biologia Plantarum, 2019 The increasing world population and limited amount of land area appropriate for intensive agriculture necessitate high-yield cultivars. The focus is on the enrichment of existing crops deficient in nutrients, which is also called biofortification ...
T. VLČKO, L. OHNOUTKOVÁ
doaj +1 more source
Exploring the transcription activator-like effectors scaffold versatility to expand the toolbox of designer nucleases [PDF]
BMC Molecular Biology, 2014 The past decade has seen the emergence of several molecular tools that render possible modification of cellular functions through accurate and easy addition, removal, or exchange of genomic DNA sequences. Among these technologies, transcription activator-like effectors (TALE) has turned out to be one of the most versatile and incredibly robust platform
Juillerat, Alexandre +11 more
openaire +3 more sources