Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples [PDF]
, 2016 Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms
Cuvelier, Claude +5 more
core +2 more sources
Evaluation of diversity, specialization, and gene specificity in transcriptomes [PDF]
, 2009 The transcriptome is a set of genes transcribed in a given tissue under specific conditions and can be characterized by a list of genes with their corresponding frequencies of transcription.
Humberto Reyes-Valdé +1 more
core +2 more sources
The Human Mitochondrial Transcriptome [PDF]
Cell, 2011 The human mitochondrial genome comprises a distinct genetic system transcribed as precursor polycistronic transcripts that are subsequently cleaved to generate individual mRNAs, tRNAs, and rRNAs. Here, we provide a comprehensive analysis of the human mitochondrial transcriptome across multiple cell lines and tissues.
Mercer, Tim R. +11 more
openaire +6 more sources
Cerebellum Transcriptome of Mice Bred for High Voluntary Activity Offers Insights into Locomotor Control and Reward-Dependent Behaviors. [PDF]
, 2016 The role of the cerebellum in motivation and addictive behaviors is less understood than that in control and coordination of movements. High running can be a self-rewarding behavior exhibiting addictive properties.
Caetano-Anollés, Kelsey +6 more
core +2 more sources
RNA-Seq analysis of splicing in Plasmodium falciparum uncovers new splice junctions, alternative splicing and splicing of antisense transcripts. [PDF]
, 2011 Over 50% of genes in Plasmodium falciparum, the deadliest human malaria parasite, contain predicted introns, yet experimental characterization of splicing in this organism remains incomplete.
DeRisi, Joseph L +2 more
core +3 more sources
Characterization of the transcriptome, nucleotide sequence polymorphism, and natural selection in the desert adapted mouse Peromyscus eremicus [PDF]
, 2014 As a direct result of intense heat and aridity, deserts are thought to be among the most harsh of environments, particularly for their mammalian inhabitants. Given that osmoregulation can be challenging for these animals, with failure resulting in death,
Eisen, Michael B, MacManes, Matthew D
core +4 more sources
BMC Genomics, 2018 Background The SOS response is an almost ubiquitous response of cells to genotoxic stresses. The full complement of genes in the SOS regulon for Vibrio species has only been addressed through bioinformatic analyses predicting LexA binding box consensus ...
Evelyne Krin +12 more
doaj +1 more source
De novo transcriptome sequencing and SSR markers development for Cedrela balansae C. DC., a native tree species of northwest Argentina [PDF]
, 2018 The endangered Cedrela balansae C.DC. (Meliaceae) is a high-value timber species with great potential for forest plantations that inhabits the tropical forests in Northwestern Argentina.
Acuña, Cintia Vanesa +11 more
core +2 more sources
Analysis of splice variants of the human protein disulfide isomerase (P4HB) gene
BMC Genomics, 2020 Background Protein Disulfide Isomerases are thiol oxidoreductase chaperones from thioredoxin superfamily with crucial roles in endoplasmic reticulum proteostasis, implicated in many diseases.
Daniela Kajihara +9 more
doaj +1 more source
Microorganisms, 2023 In Ruminiclostridium cellulolyticum, cellobiose is imported by the CuaABC ATP-binding cassette transporter containing the solute-binding protein (SBP) CuaA and is further degraded in the cytosol by the cellobiose phosphorylase CbpA.
Aurélie Fosses +7 more
doaj +1 more source