Results 61 to 70 of about 451,072 (262)

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

CapTrap-seq: a platform-agnostic and quantitative approach for high-fidelity full-length RNA sequencing

Nature Communications
Long-read RNA sequencing is essential to produce accurate and exhaustive annotation of eukaryotic genomes. Despite advancements in throughput and accuracy, achieving reliable end-to-end identification of RNA transcripts remains a challenge for long-read ...
Sílvia Carbonell-Sala, Tamara Perteghella, Julien Lagarde, Hiromi Nishiyori, Emilio Palumbo, Carme Arnan, Hazuki Takahashi, Piero Carninci, Barbara Uszczynska-Ratajczak, Roderic Guigó   +9 more
doaj   +1 more source

High-Frequency Promoter Firing Links THO Complex Function to Heavy Chromatin Formation

Cell Reports, 2013
The THO complex is involved in transcription, genome stability, and messenger ribonucleoprotein (mRNP) formation, but its precise molecular function remains enigmatic.
John Mouaikel, Sébastien Z. Causse, Mathieu Rougemaille, Yves Daubenton-Carafa, Corinne Blugeon, Sophie Lemoine, Frédéric Devaux, Xavier Darzacq, Domenico Libri   +8 more
doaj   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Transcriptome and Temporal Transcriptome Analyses in Single Cells

International Journal of Molecular Sciences
Transcriptome analysis in single cells, enabled by single-cell RNA sequencing, has become a prevalent approach in biomedical research, ranging from investigations of gene regulation to the characterization of tissue organization. Over the past decade, advances in single-cell RNA sequencing technology, including its underlying chemistry, have ...
Jun Lyu, Chongyi Chen
openaire   +2 more sources

Methodology of DNA extraction and sequencing from living cardiomyocytes collected by catheter in humans

Genetics in Medicine Open
Purpose: We present here the technical feasibility of percutaneously retrieving cardiomyocytes (CMs) through the lumen of irrigated ablation catheters, with the aim of obtaining DNA of sufficient quality/quantity for allowing DNA amplification, screening,
Flavie Ader, Céline Guilbeau-Frugier, Emeline Lhuillier, Frédéric Martins, Anne Alicia Gonzalez, Anne Rollin, Maxime Beneyto, Céline Gales, Filipe Pires, Jean-José Maoret, Jean-Michel Sénard, Jean Timnou-Bekouti, Eric Villard, Laetitia Duboscq-Bidot, Estelle Gandjbakhch, Philippe Maury   +15 more
doaj   +1 more source

Gene Expression Profile Related to the Progression of Preneoplastic Nodules toward Hepatocellular Carcinoma in Rats

Neoplasia: An International Journal for Oncology Research, 2006
In this study, we investigated the time course gene expression profile of preneoplastic nodules and hepatocellular carcinomas (HCC) to define the genes implicated in cancer progression in a resistant hepatocyte model. Tissues that included early nodules (
Julio Isael Pérez-Carréon, Cristina López-García, Samia Fattel-Fazenda, Evelia Arce-Popoca, Leticia Alemán-Lazarini, Sergio Hernández-García, Véronique Le Berre, Sergueï Sokol, Jean Marie Francois, Saúl Villa-Treviño   +9 more
doaj   +1 more source