Results 151 to 160 of about 851,785 (340)

Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system

, 2022
Camilo Perez, Anahí Sanluis‐Verdes, Ariel Waisman, Antonella Lombardi, Gustavo Rosero, Alejandro La Greca, Shekhar Bhansali, Natalia Bourguignon, Carlos Luzzani, Maximiliano Pérez, Santiago Miriuka, Betiana Lerner   +11 more
openalex   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Effect of using amino acids in the freeze‑drying of siRNA lipoplexes on gene knockdown in cells after reverse transfection

, 2021
Min Tang, Yoshiyuki Hattori
openalex   +2 more sources

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

FEBS Open Bio, EarlyView.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru, Deepak Kumar Mishra, Shiva Senthil Kumar, Hao‐Han Pang, Brendan Devine, Komal Khan, Rachid Drissi   +6 more
wiley   +1 more source

Modification of tumor cell exosome content by transfection with wt-p53 and microRNA-125b expressing plasmid DNA and its effect on macrophage polarization [PDF]

, 2016
Meghana V. Trivedi, Meghna Talekar, Parin Shah, Qing Ouyang, Mansoor M. Amiji   +4 more
openalex   +1 more source

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source

Optimized conditions for successful transfection of human endothelial cells with in vitro synthesized and modified mRNA for induction of protein expression [PDF]

, 2014
Meltem Avci‐Adali, Andreas Behring, Timea Keller, Stefanie Krajewski, Christian Schlensak, Hans Peter Wendel   +5 more
openalex   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Transfection of insect cell in suspension for efficient baculovirus generation [PDF]

, 2016
Susan Roest, Sandra Kapps-Fouthier, Julia Klopp, S. Rieffel, Bernd Gerhartz, Bikram Prasad Shrestha   +5 more
openalex   +1 more source

Nanoscale Membrane Pores Follow Two Tension-Triggered Pathways to Membrane Rupture During Exocytosis, Drug Delivery and Transfection [PDF]

, 2021
Dong An, Sathish Thiyagarajan, Egor Antipov, Brett E. Alcott, Ben O’Shaughnessy   +4 more
openalex   +1 more source