Results 91 to 100 of about 8,555 (214)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Characterization of Three-Dimensional Strong Force Chain Properties of Mineral Aggregate Mixtures Based on the Discrete Element Method

Buildings
The skeleton structure composed of mineral aggregates is the main body to bear and transfer external loading in asphalt mixtures. To investigate the loading transfer mechanism of the mineral aggregate skeleton, the uniaxial penetration test and Discrete ...
Yuan Gao, Guoqiang Liu, Nan Jiang
doaj   +1 more source

Modeling Heat Transfer for Assessing the Convection Length in Ventilated Caves

Journal of Geophysical Research: Earth Surface
AbstractThe present study focuses on heat transfer in ventilated caves for which the airflow is driven by the temperature contrast between the cave and the external atmosphere. We use a numerical model that couples the convective heat transfer due to the airflow in a single karst conduit with the conductive heat transfer in the rock mass.
Sedaghatkish, Amir, Pastore, Claudio, Doumenc, Frédéric, Jeannin, Pierre‐yves, Luetscher, Marc   +4 more
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Mathematical Simulation of Graphene With Modified c-c Bond Length and Transfer Energy [PDF]

Журнал нано- та електронної фізики, 2011
In nanotechnology research, allotropes of carbon like Graphene, Fullerene (Buckyball) and Carbon nanotubes are widely used due to their remarkable properties.
P.A. Alvi, S.Z. Hashmi, S. Dalela, F. Rahman   +3 more
doaj  

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Enhancing heat transfer in compound twisted square ducts using shortened twisted tape inserts

Results in Engineering
Enhancing heat transfer is an important area in various forms of energy conservation that is closely related to economically efficient systems. A typical technique for improving heat transfer in thermal systems is the use of tape inserts. The majority of
Chithra V P, Balaji Bakthavatchalam, V. Jayakumar, Sambhaji Kusekar, A.K. Pandey, Khairul Habib, Salem Algarni, Talal Alqahtani   +7 more
doaj   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source