Results 61 to 70 of about 305,705 (319)

Ultra-fast conductive media for RNA electrophoretic mobility shift assays

BioTechniques, 2020
The use of RNA electrophoretic mobility shift assays (REMSAs) for analysis of RNA–protein interactions have been limited to lengthy assay time and qualitative assessment.
Samantha Z Brown, Lebaron C Agostini, Henry L Thomsett, Jonathan R Brody   +3 more
doaj   +1 more source

Profiling Selective Packaging of Host RNA and Viral RNA Modification in SARS-CoV-2 Viral Preparations

Frontiers in Cell and Developmental Biology, 2022
Viruses package host RNAs in their virions which are associated with a range of functions in the viral life cycle. Previous transcriptomic profiling of host RNA packaging mostly focused on retroviruses.
Noah Peña, Wen Zhang, Christopher Watkins, Mateusz Halucha, Hala Alshammary, Matthew M. Hernandez, Matthew M. Hernandez, Wen-Chun Liu, Wen-Chun Liu, Randy A. Albrecht, Randy A. Albrecht, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Viviana Simon, Viviana Simon, Viviana Simon, Viviana Simon, Christopher Katanski, Tao Pan, Tao Pan   +21 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences. [PDF]

, 2020
Hematopoietic stem cell gene therapy is a promising approach for treating disorders of the hematopoietic system. Identifying combinations of cis-regulatory elements that do not impede packaging or transduction efficiency when included in lentiviral ...
Aleshe, Bamidele, Ayoub, Paul George, Brown, Devin, Hollis, Roger P, Kohn, Donald B, Kurita, Ryo, Lathrop, Lindsay, Ma, Feiyang, Morgan, Richard A, Nakamura, Yukio, Pellegrini, Matteo, Senadheera, Shantha, Tam, Curtis, Unti, Mildred J, Wong, Ryan L   +14 more
core   +1 more source

Hyaluronan and Hyaluronidase, which is better for embryo development? [PDF]

, 2016
Our aim was to examine size-specific effects of Hyaluronan (HA) on preimplantation embryo development. We investigated the effects of Hyalovet (HA, 500–750 kDa; the size produced by HA synthase-3, which is abundant in the oviduct), or HA treated with ...
Ali A. Fouladi-Nashta, Aruffo, Aviles, Bastow, Bergqvist, Block, Bourguignon, Choudhary, Csoka, Dicker, Fenwick, Fouladi-Nashta, Furnus, Furnus, Georgiou, Hahnel, Hess, Kabir A. Raheem, Lane, Laurent, Lesley, Lloyd, Marei, Marei, Mariani, Matwee, Mazdak Salavati, Mehta, Mohey-Elsaeed, Muhammad Khalid, Neira, Oesterreicher, Ohno-Nakahara, Pandey, Pushpakumara, Raheem, Robinson, Rozen, Russell, Ryan, Saliba, Schmidt, Seytanoglu, Stern, Stevenson, Tienthai, Tina Tremaine, Tombolan, Toole, Torner, Ulbrich, Waleed F.A. Marei, Wang, Wathes, Watson, Way, Weigel, Xu, Yevdokimova, Yoon   +59 more
core   +2 more sources

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

Profiling of RNAs from Human Islet-Derived Exosomes in a Model of Type 1 Diabetes [PDF]

, 2019
Type 1 diabetes (T1D) is characterized by the immune-mediated destruction of insulin-producing islet β cells. Biomarkers capable of identifying T1D risk and dissecting disease-related heterogeneity represent an unmet clinical need.
Evans-Molina, Carmella, G. Mirmira, Raghavendra, Jiyun Kang, Nicole, Krishnan, Preethi, Syed, Farooq   +4 more
core   +1 more source

tiRNAs: Insights into Their Biogenesis, Functions, and Future Applications in Livestock Research

Non-Coding RNA, 2022
Transfer RNA (tRNA)-derived small RNAs (tsRNAs) belong to a group of transfer ribonucleic acid (tRNA)-derived fragments that have recently gained interest as molecules with specific biological functions.
Fabio Sarais, Alvaro Perdomo-Sabogal, Klaus Wimmers, Siriluck Ponsuksili   +3 more
doaj   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Microfluidic DNA amplification - a review [PDF]

, 2009
The application of microfluidic devices for DNA amplification has recently been extensively studied. Here, we review the important development of microfluidic polymerase chain reaction (PCR) devices and discuss the underlying physical principles for the ...
Ozdemir, Pinar, Zhang, Yonghao
core   +1 more source