Results 41 to 50 of about 159,938 (238)

Small RNA populations revealed by blocking rRNA fragments in Drosophila melanogaster reproductive tissues. [PDF]

PLoS ONE, 2018
RNA interference (RNAi) is a complex and highly conserved regulatory mechanism mediated via small RNAs (sRNAs). Recent technical advances in high throughput sequencing have enabled an increasingly detailed analysis of sRNA abundances and profiles in ...
Emily K Fowler, Irina Mohorianu, Damian T Smith, Tamas Dalmay, Tracey Chapman   +4 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Non-coding RNAs and epithelial mesenchymal transition in cancer: molecular mechanisms and clinical implications

Journal of Experimental & Clinical Cancer Research, 2022
Epithelial-mesenchymal transition (EMT) is a fundamental process for embryonic development during which epithelial cells acquire mesenchymal characteristics, and the underlying mechanisms confer malignant features to carcinoma cells such as dissemination
Hashem Khanbabaei, Saeedeh Ebrahimi, Juan Luis García-Rodríguez, Zahra Ghasemi, Hossein Pourghadamyari, Milad Mohammadi, Lasse Sommer Kristensen   +6 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Ultra-fast conductive media for RNA electrophoretic mobility shift assays

BioTechniques, 2020
The use of RNA electrophoretic mobility shift assays (REMSAs) for analysis of RNA–protein interactions have been limited to lengthy assay time and qualitative assessment.
Samantha Z Brown, Lebaron C Agostini, Henry L Thomsett, Jonathan R Brody   +3 more
doaj   +1 more source

Profiling Selective Packaging of Host RNA and Viral RNA Modification in SARS-CoV-2 Viral Preparations

Frontiers in Cell and Developmental Biology, 2022
Viruses package host RNAs in their virions which are associated with a range of functions in the viral life cycle. Previous transcriptomic profiling of host RNA packaging mostly focused on retroviruses.
Noah Peña, Wen Zhang, Christopher Watkins, Mateusz Halucha, Hala Alshammary, Matthew M. Hernandez, Matthew M. Hernandez, Wen-Chun Liu, Wen-Chun Liu, Randy A. Albrecht, Randy A. Albrecht, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Viviana Simon, Viviana Simon, Viviana Simon, Viviana Simon, Christopher Katanski, Tao Pan, Tao Pan   +21 more
doaj   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

The MIME-seq technique allows to monitor the interaction of small non-coding RNAs with Argonaute proteins and their transfer to other cells

Scientific Reports
The MIME-seq2.0 technique utilizes a chimeric methyltransferase engineered to bind Argonaute proteins (hereafter referred to MIME enzyme) to protect the terminal sugar of small non-coding RNAs (sncRNAs) from oxidation, a reaction that inhibits unmodified
Jérôme Perrard, Claudiane Guay, Nadège Zanou, Romano Regazzi   +3 more
doaj   +1 more source