Single-molecule studies of the dynamics and interactions of bacterial OXPHOS complexes [PDF]
Although significant insight has been gained into biochemical, genetic and structural features of oxidative phosphorylation (OXPHOS) at the single-enzyme level, relatively little was known of how the component complexes function together in time and space until recently.
arxiv
Molecular biology on a microfluidic chip [PDF]
We have developed microfluidic chips for automating molecular biology processes such as gene ligation and gene transformation from nanolitre sample volumes with parallel architecture. Unlike conventional tube methods with cumbersome pipetting procedures,
Anderson, W. French+3 more
core +1 more source
Boundaries of photosynthesis: adaptations of carbon fixation in extreme environments
Photosynthesis faces challenges from environmental extremes of temperature, pH, and salinity, limiting gas diffusion, modifying membrane fluidity, and destabilizing photochemical and biochemical reactions. Photosynthetic organisms have evolved unique adaptations overcoming these stresses and maintaining their photosynthetic activity.
Pere Aguiló‐Nicolau+3 more
wiley +1 more source
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna+4 more
wiley +1 more source
Characterization of the role that bacterial surface polysaccharide poly N-acetyl glucosamine plays in nonvaccine serotypes of streptococcus pneumoniae colonization and pathogenicity [PDF]
Poly N-acetyl glucosamine is a cell surface polysaccharide that has been characterized in Staphylococcus epidermidis and Staphylococcus aureus as involved in biofilm formation and implicated in virulence.
Jiang, Fanny
core +1 more source
Understanding and Overcoming Immunotherapy Resistance in Skin Cancer: Mechanisms and Strategies
This narrative review explores the mechanisms driving immunotherapy resistance in skin cancer, including tumor microenvironment factors, genetic mutations, and immune evasion strategies. It highlights potential strategies to overcome resistance, offering insights for improving therapeutic outcomes and guiding future research in personalized ...
Shreya Singh Beniwal+8 more
wiley +1 more source
T-DNA promoter tagging in Nicotiana tabacum : a thesis presented in fulfilment of the requirements for the degree of Master of Philosophy in Genetics at Massey University, Palmerston North, New Zealand [PDF]
Plant development is primarily controlled at the level of gene expression. In order to analyse this regulation it is necessary to isolate genes which are involved in organ development through cellular and tissue determination or which respond to ...
Kerr, Donald Haldane
core
The Guanylate Cyclase C-cGMP Signaling Axis Opposes Intestinal Epithelial Injury and Neoplasia. [PDF]
Guanylate cyclase C (GUCY2C) is a transmembrane receptor expressed on the luminal aspect of the intestinal epithelium. Its ligands include bacterial heat-stable enterotoxins responsible for traveler\u27s diarrhea, the endogenous peptide hormones ...
Rappaport, Jeffrey A., Waldman, Scott A.
core +2 more sources
Bacterial Evolution: Dynamic Genomes and the Power of Transformation [PDF]
Virulence and avirulence genes carried on large, unstable pathogenicity islands (PAI) strongly influence the course and fate of host-pathogen interactions. A recent study shows how one such PAI can be rapidly transferred between two closely related bacteria via transformation in vivo, and how this horizontal gene transfer affects the fitness of the ...
openaire +3 more sources
We have developed a simple, one-step procedure for the preparation of competent Escherichia coli that uses a transformation and storage solution [TSS; 1 x TSS is LB broth containing 10% (wt/vol) polyethylene glycol, 5% (vol/vol) dimethyl sulfoxide, and ...
C. Chung+2 more
semanticscholar +1 more source