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Bacterial Transformation (Kushner Method)

2003
The principle of this procedure is very similar to that of the standard calcium chloride method, (see Chapter 34 ) i.e., exponential phase cells are harvested and washed with a solution containing divalent cations. This renders the cells competent, which simply means they are now able to take up DNA from the solution.
J W, Dale, P J, Greenaway
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