Results 51 to 60 of about 3,602,698 (355)

Transgene-free genome editing in marine algae by bacterial conjugation – comparison with biolistic CRISPR/Cas9 transformation

open access: yesScientific Reports, 2018
The CRISPR/Cas9 technology has opened the possibility for targeted genome editing in various organisms including diatom model organisms. One standard method for delivery of vectors to diatom cells is by biolistic particle bombardment.
Amit Kumar Sharma   +4 more
semanticscholar   +1 more source

Seasonal changes in microbial dissolved organic sulfur transformations in coastal waters [PDF]

open access: yes, 2020
The marine trace gas dimethylsulfide (DMS) is the single most important biogenic source of atmospheric sulfur, accounting for up to 80% of global biogenic sulfur emissions.
Dixon, Joanna L.   +3 more
core   +1 more source

Integrated FT-ICR MS and metabolome reveals diatom-derived organic matter by bacterial transformation under warming and acidification

open access: yesiScience, 2023
Summary: Bacterial transformation and processing of diatom-derived organic matter (OM) is extremely important for the cycling of production and energy in marine ecosystems; this process contributes to the production of microbial food webs. In this study,
Yang Liu, Chao Ma, Jun Sun
doaj  

Label-free identification of individual bacteria using Fourier transform light scattering [PDF]

open access: yes, 2015
Rapid identification of bacterial species is crucial in medicine and food hygiene. In order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an optical method based on Fourier transform light scattering (FTLS) measurements and statistical classification.
arxiv   +1 more source

Ethylene production in plants during transformation suppresses vir gene expression in Agrobacterium tumefaciens [PDF]

open access: yes, 2008
•Ethylene evolution from plants inhibits Agrobacterium-mediated genetic transformation, but the mechanism is little understood. In this study, the possible role of ethylene in Agrobacterium-mediated genetic transformation was clarified.•It was tested ...
Ezura Hiroshi   +6 more
core   +1 more source

Transformation Asymmetry and the Evolution of the Bacterial Accessory Genome [PDF]

open access: yesMolecular Biology and Evolution, 2017
Bacterial transformation can insert or delete genomic islands (GIs), depending on the donor and recipient genotypes, if an homologous recombination spans the GI's integration site and includes sufficiently long flanking homologous arms. Combining mathematical models of recombination with experiments using pneumococci found GI insertion rates declined ...
Apagyi, K, Fraser, C, Croucher, N
openaire   +6 more sources

Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria

open access: yesNature Communications, 2022
ComEA is a DNA-binding protein required for DNA uptake during bacterial transformation. Here, Ahmed et al. determine X-ray crystal structures of ComEA from Gram-positive bacteria, identifying a domain that is absent in Gram-negative bacteria and drives ...
Ishtiyaq Ahmed   +6 more
doaj   +1 more source

Modelling of Indicator Escherichia coli Contamination in Sentinel Oysters and Estuarine Water. [PDF]

open access: yes, 2019
This study was performed to improve the ability to predict the concentrations of Escherichia coli in oyster meat and estuarine waters by using environmental parameters, and microbiological and heavy metal contamination from shellfish growing area in ...
Atwill, Edward R   +1 more
core   +2 more sources

Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of Streptococcus pneumoniae Associated with Distinct Alleles of the Type I Restriction-Modification System. [PDF]

open access: yes, 2020
The capsule is the dominant Streptococcus pneumoniae virulence factor, yet how variation in capsule thickness is regulated is poorly understood. Here, we describe an unexpected relationship between mutation of adcAII, which encodes a zinc uptake ...
Arioli   +51 more
core   +3 more sources

Mechanism of transformation in Mycobacteria using a novel shockwave assisted technique driven by in-situ generated oxyhydrogen

open access: yesScientific Reports, 2017
We present a novel method for shockwave-assisted bacterial transformation using a miniature oxyhydrogen detonation-driven shock tube. We have obtained transformation efficiencies of about 1.28 × 106, 1.7 × 106, 5 × 106, 1 × 105, 1 × 105 and 2 × 105 ...
Akshay Datey   +3 more
doaj   +1 more source

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