Results 51 to 60 of about 3,602,698 (355)
The CRISPR/Cas9 technology has opened the possibility for targeted genome editing in various organisms including diatom model organisms. One standard method for delivery of vectors to diatom cells is by biolistic particle bombardment.
Amit Kumar Sharma+4 more
semanticscholar +1 more source
Seasonal changes in microbial dissolved organic sulfur transformations in coastal waters [PDF]
The marine trace gas dimethylsulfide (DMS) is the single most important biogenic source of atmospheric sulfur, accounting for up to 80% of global biogenic sulfur emissions.
Dixon, Joanna L.+3 more
core +1 more source
Summary: Bacterial transformation and processing of diatom-derived organic matter (OM) is extremely important for the cycling of production and energy in marine ecosystems; this process contributes to the production of microbial food webs. In this study,
Yang Liu, Chao Ma, Jun Sun
doaj
Label-free identification of individual bacteria using Fourier transform light scattering [PDF]
Rapid identification of bacterial species is crucial in medicine and food hygiene. In order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an optical method based on Fourier transform light scattering (FTLS) measurements and statistical classification.
arxiv +1 more source
Ethylene production in plants during transformation suppresses vir gene expression in Agrobacterium tumefaciens [PDF]
•Ethylene evolution from plants inhibits Agrobacterium-mediated genetic transformation, but the mechanism is little understood. In this study, the possible role of ethylene in Agrobacterium-mediated genetic transformation was clarified.•It was tested ...
Ezura Hiroshi+6 more
core +1 more source
Transformation Asymmetry and the Evolution of the Bacterial Accessory Genome [PDF]
Bacterial transformation can insert or delete genomic islands (GIs), depending on the donor and recipient genotypes, if an homologous recombination spans the GI's integration site and includes sufficiently long flanking homologous arms. Combining mathematical models of recombination with experiments using pneumococci found GI insertion rates declined ...
Apagyi, K, Fraser, C, Croucher, N
openaire +6 more sources
ComEA is a DNA-binding protein required for DNA uptake during bacterial transformation. Here, Ahmed et al. determine X-ray crystal structures of ComEA from Gram-positive bacteria, identifying a domain that is absent in Gram-negative bacteria and drives ...
Ishtiyaq Ahmed+6 more
doaj +1 more source
Modelling of Indicator Escherichia coli Contamination in Sentinel Oysters and Estuarine Water. [PDF]
This study was performed to improve the ability to predict the concentrations of Escherichia coli in oyster meat and estuarine waters by using environmental parameters, and microbiological and heavy metal contamination from shellfish growing area in ...
Atwill, Edward R+1 more
core +2 more sources
Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of Streptococcus pneumoniae Associated with Distinct Alleles of the Type I Restriction-Modification System. [PDF]
The capsule is the dominant Streptococcus pneumoniae virulence factor, yet how variation in capsule thickness is regulated is poorly understood. Here, we describe an unexpected relationship between mutation of adcAII, which encodes a zinc uptake ...
Arioli+51 more
core +3 more sources
We present a novel method for shockwave-assisted bacterial transformation using a miniature oxyhydrogen detonation-driven shock tube. We have obtained transformation efficiencies of about 1.28 × 106, 1.7 × 106, 5 × 106, 1 × 105, 1 × 105 and 2 × 105 ...
Akshay Datey+3 more
doaj +1 more source