Results 51 to 60 of about 3,877,619 (352)

Markerless gene deletion in Ralstonia solanacearum based on its natural transformation competence

Frontiers in Microbiology, 2022
Ralstonia solanacearum species complex (RSSC) is a group of Gram-negative bacterial pathogen capable of infecting numerous plants and crops, causing severe vascular wilt diseases.
Jinli Yan, Jinli Yan, Nuoqiao Lin, Nuoqiao Lin, Xiaoqing Wang, Xiaoqing Wang, Xuemei Chen, Xuemei Chen, Huishan Wang, Huishan Wang, Qiqi Lin, Qiqi Lin, Xiaofan Zhou, Lianhui Zhang, Lianhui Zhang, Lisheng Liao   +15 more
doaj   +1 more source

Antimicrobial surfaces based on self-assembled nanoreactors : from block copolymer synthesis to bacterial adhesion study [PDF]

, 2010
The aim of this work is to develop a new strategy for the prevention of biofilm growth. For this purpose, we prepared bioactive surfaces resulting from the surface-immobilization of nanoreactors self-assembled from amphiphilic poly(isobutylene)-block ...
Cottenye, Nicolas
core   +1 more source

A Fast and Practical Yeast Transformation Method Mediated by Escherichia coli Based on a Trans-Kingdom Conjugal Transfer System: Just Mix Two Cultures and Wait One Hour. [PDF]

PLoS ONE, 2016
Trans-kingdom conjugation is a phenomenon by which DNA is transferred into a eukaryotic cell by a bacterial conjugal transfer system. Improvement in this method to facilitate the rapid co-cultivation of donor bacterial and recipient eukaryotic cell ...
Kazuki Moriguchi, Shinji Yamamoto, Yuta Ohmine, Katsunori Suzuki   +3 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Metagenomic Insights into How Understory Vegetation Enhances Soil Nitrogen Availability via Microbial Nitrogen Transformation in Poplar Plantations

Agronomy
Long-term monoculture of poplar plantations for industrial material production has been widely reported to cause severe soil degradation, while the presence of understory vegetation might enhance soil nitrogen (N) transformation and supply.
Wenyu Jia, Tong Li, Peilei Ye, Yuxin Chen, Ruoning Zhu, Ruixin Yan, Haoran Yue, Ye Tian   +7 more
doaj   +1 more source

Mechanism of transformation in Mycobacteria using a novel shockwave assisted technique driven by in-situ generated oxyhydrogen

Scientific Reports, 2017
We present a novel method for shockwave-assisted bacterial transformation using a miniature oxyhydrogen detonation-driven shock tube. We have obtained transformation efficiencies of about 1.28 × 106, 1.7 × 106, 5 × 106, 1 × 105, 1 × 105 and 2 × 105 ...
Akshay Datey, Janardhanraj Subburaj, Jagadeesh Gopalan, Dipshikha Chakravortty   +3 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Defining bacterial species in the genomic era : insights from the genus Acinetobacter [PDF]

, 2012
Background: Microbial taxonomy remains a conservative discipline, relying on phenotypic information derived from growth in pure culture and techniques that are time-consuming and difficult to standardize, particularly when compared to the ease of modern
Loman, Nicholas J, Chan, Jacqueline, Nicholas J Loman, Chan, Jacqueline Z-m, Constantinidou, Chrystala, Loman Nicholas J, Halachev, Mihail R, Pallen, Mark J, Pallen, Mark J., Halachev Mihail R, Mihail R Halachev, Loman, Nicholas J., Pallen Mark J, Halachev, Mihail R., Jacqueline Z-M Chan, Chrystala Constantinidou, Chan Jacqueline Z-M, Constantinidou Chrystala, Mark J Pallen   +18 more
core   +1 more source

Bacterial bioprinting on a flexible substrate for fabrication of a colorimetric temperature indicator by using a commercial inkjet printer

Journal of Medical Signals and Sensors, 2018
Background: Bacterial sensors are recommended for medical sciences, pharmaceutical industries, food industries, and environmental monitoring due to low cost, high sensitivity, and appropriate response time.
Zeinab Mohammadi, Mohsen Rabbani
doaj   +1 more source

Bacterial transformations of 1,2,3,4-tetrahydrodibenzothiophene and dibenzothiophene [PDF]

Applied and Environmental Microbiology, 1997
The transformations of 1,2,3,4-tetrahydrodibenzothiophene (THDBT) were investigated with pure cultures of hydrocarbon-degrading bacteria. Metabolites were extracted from cultures with dichloromethane (DCM) and analyzed by gas chromatography (GC) with flame photometric, mass, and Fourier transform infrared detectors.
K G, Kropp, J T, Andersson, P M, Fedorak
openaire   +2 more sources