Results 81 to 90 of about 470,402 (305)
The role of the N-acetylglucosamine phosphoenolpyruvate phosphotransferase system from Lactobacillus plantarum 8014 in the mechanism of action of glycocin F : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University, Manawatū, New Zealand [PDF]
, 2017 The rise in antibiotic-resistant bacteria is becoming a severe public health problem because of the shortage of new antibiotics to combat existing resistant bacterial pathogens.
Bailie, Marc Alex
core
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Methods in Ecology and EvolutionThe gut microbiota is increasingly recognized as a rich source of biological data that offers critical information on host health, including information on pathological conditions such as cancer, diabetes and other metabolic disorders.
David Martin +3 more
doaj +1 more source
Selective detection of bacterial layers with terahertz plasmonic antennas [PDF]
, 2012 Current detection and identification of micro-organisms is based on either rather unspecific rapid microscopy or on more accurate complex, time-consuming procedures.
Abbas +16 more
core +2 more sources
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Chlamydial infection from outside to inside [PDF]
, 2019 Chlamydia are obligate intracellular bacteria, characterized by a unique biphasic developmental cycle. Specific interactions with the host cell are crucial for the bacteria's survival and amplification because of the reduced chlamydial genome.
Gitsels, Arlieke +2 more
core +2 more sources
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE
Scientific Papers Animal Science and Biotechnologies, 2023 An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL +2 more
doaj
Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]
, 2012 Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA +4 more
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source