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2011
Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
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Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
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Genetic transformation of fungi
The International Journal of Developmental Biology, 2017Transferring DNA into cells is an essential research method for molecular cloning and gene function studies. As molecular biology and materials physics develop, more and more new transformation methods have been applied to mammalian cells. Some techniques have been successfully developed for several types of fungi, but their efficiencies are extremely ...
Liya, He +7 more
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Tubercle, 1989
Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
L, Lugosi, W R, Jacobs, B R, Bloom
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Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
L, Lugosi, W R, Jacobs, B R, Bloom
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Coffea SPP. Genetic Transformation
2004Selon les auteurs, le besoin d'une méthode satisfaisante de transformation génétique du caféier est très important, à la fois pour évaluer la fonctionnalité des gènes, et pour introduire de nouveaux gènes intéressants dans des génotypes d'élite de caféier. En partant d'un protocole existant, ils ont développé leur propre procédure à haut rendement, qui
Leroy, Thierry, Dufour, Magali
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Genetic transformation ofPelargonium X hortorum
Plant Cell Reports, 1995TransgenicPelargonium X hortorum have been producedvia Agrobacterium tumefaciens-mediated transformation. The regeneration protocol used provided a regeneration frequency approximately to 95 percent. Clumps of regenerants, from cotyledons and hypocotyls ofPelargonium X hortorum seedlings, were inoculated with the disarmed strain EHA101 ofAgrobacterium ...
Robichon, M.P. +2 more
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Genetic Transformation of Switchgrass
2009Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used
Yajun, Xi, Yaxin, Ge, Zeng-Yu, Wang
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Genetic transformation of grapevine cells
Plant Cell Reports, 1989Biovar 1 strains ofAgrobacterium tumefaciens have been used to transform a cell suspension culture ofVitis vinifera cv. Cabernet Sauvignon. Cocultivation of cultures withAgrobacterium strains bearing either the cointegrate pGV3850::1103neo, or the binary vector pGA474-68, each gave rise to kanamycin resistant tissue.
T J, Baribault +2 more
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