Results 271 to 280 of about 1,102,835 (325)
Genome editing in mouse spermatogonial stem cell lines targeting the Tex15 gene using CRISPR/Cas9. [PDF]
Front Vet SciYeşilbostan S +4 more
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Precise measurement of molecular phenotypes with barcode-based CRISPRi systems. [PDF]
Genome BiolLobel JH, Ingolia NT.
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A tRNA-gRNA multiplexing system for CRISPR genome editing inMarchantia polymorpha
Frangedakis E +6 more
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Comparative performance of novel viral landscape phylogeography approaches
Dellicour S +8 more
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Genetic transformation of fungi
The International Journal of Developmental Biology, 2017Transferring DNA into cells is an essential research method for molecular cloning and gene function studies. As molecular biology and materials physics develop, more and more new transformation methods have been applied to mammalian cells. Some techniques have been successfully developed for several types of fungi, but their efficiencies are extremely ...
Chunmei Chen +7 more
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Tubercle, 1989
Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
Barry R. Bloom +2 more
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Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
Barry R. Bloom +2 more
openaire +3 more sources
2011
Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
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Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
openaire +1 more source
Genetic transformation of mycobacteria [PDF]
Trends in Microbiology, 1993 Recent advances in methods for introducing DNA into both fast- and slow-growing species of mycobacteria have stimulated the construction of a variety of cloning vectors and the development of a versatile genetic system. These tools will facilitate studies of mycobacterial pathogenicity, antibiotic action and drug resistance, and lead to more effective ...
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Genetic Transformation of Switchgrass
2009Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used
Zeng-Yu Wang, Yajun Xi, Yaxin Ge
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Genetics of drug transformation
Clinical Biochemistry, 1986Biotransformations of drugs are controlled or strongly affected by genetic factors. During the past few years several genetic deficiencies of drug-metabolizing reactions catalyzed by members of the family of cytochrome P-450 were observed. Choice of the appropriate drug to study and attention to urinary metabolites have been the essential ingredients ...
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