Results 271 to 280 of about 5,482,088 (343)

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

open access: yesFEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo   +4 more
wiley   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Studies on the mother flower carnation: past, present, and future. [PDF]

open access: yesHortic Res
Wang M   +7 more
europepmc   +1 more source

Haploid Production in <i>Cannabis sativa</i>: Recent Updates, Prospects, and Perspectives. [PDF]

open access: yesBiology (Basel)
Ahsan SM   +6 more
europepmc   +1 more source

Missense Mutations in the KAT Domain of <i>CREBBP</i> Gene in Patients with Follicular Lymphoma: Implications for Differential Diagnosis and Prognosis. [PDF]

open access: yesInt J Mol Sci
Smolianinova A   +13 more
europepmc   +1 more source

Genetic transformation of fungi

The International Journal of Developmental Biology, 2017
Transferring DNA into cells is an essential research method for molecular cloning and gene function studies. As molecular biology and materials physics develop, more and more new transformation methods have been applied to mammalian cells. Some techniques have been successfully developed for several types of fungi, but their efficiencies are extremely ...
Chunmei Chen   +7 more
openaire   +2 more sources

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