Results 31 to 40 of about 451,048 (262)

Establishment of a Rapid and Effective Agrobacterium-Mediated Genetic Transformation System of Oxalis triangularis ‘Purpurea’

Plants, 2023
Oxalis triangularis ‘Purpurea’ has significant ornamental value in landscaping. There is a critical necessity to elucidate the gene functions of O. triangularis ‘Purpurea’ and dissect the molecular mechanisms governing key ornamental traits.
Yun Xiao, Wanli Tuo, Xuexuan Wang, Baomin Feng, Xinyu Xu, Sagheer Ahmad, Junwen Zhai, Donghui Peng, Shasha Wu   +8 more
doaj   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Leaf rust resistance in wheat and interpretation of the antifungal activity of silver and copper nanoparticles

Scientific Reports
Wheat production is jeopardized by Puccinia triticina, the pathogen responsible for wheat leaf rust. This study assessed the impact of silver (Ag) and copper (Cu) nanoparticles (NPs) on the control of wheat leaf rust disease and the underlying mechanisms
Atwa A. Atwa, Shreen S. Ahmed, Gehan H. Abd El-Aziz, Mohamed A. Abou-Zeid, Reda I. Omara, Nourhan A. Atwa, Ashraf H. Fahmy   +6 more
doaj   +1 more source

Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward

Plants, 2020
Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established.
Angela Ricci, Silvia Sabbadini, Humberto Prieto, Isabel MG Padilla, Chris Dardick, Zhijian Li, Ralph Scorza, Cecilia Limera, Bruno Mezzetti, Margarita Perez-Jimenez, Lorenzo Burgos, Cesar Petri   +11 more
doaj   +1 more source

A highly efficient genetic transformation system for broccoli and subcellular localization

Frontiers in Plant Science, 2023
IntroductionAgrobacterium-mediated genetic transformation has been widely used for the identification of functional genes and regulatory and developmental mechanisms in plants.
Yongyu Zhao, Dongxu Yang, Yumei Liu, Fengqing Han, Zhansheng Li   +4 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Agrobacterium-mediated genetic transformation of gramineous forages

上海师范大学学报. 自然科学版, 2021
The establishment of a genetic transformation system for gramineous forages is of great significance to the utilization of germplasm resources. In recent years, the genetic transformation methods of various gramineous forages such as Eremochloa ...
ZHANG Yue, LI Ying, DAI Shaojun
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Genome-wide identification and evolutionary analysis of the AP2/EREBP, COX and LTP genes in Zea mays L. under drought stress

Scientific Reports
AP2 (APETALA2)/EREBP (ethylene-responsive element-binding protein), cytochrome c oxidase (COX) and nonspecific lipid transfer proteins (LTP) play important roles in the response to drought stress.
Amaal Maghraby, Mohamed Alzalaty
doaj   +1 more source