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Background Agrobacterium tumefaciens-mediated leaf disc genetic transformation is an important way to achieve transgenics or gene editing. Ensuring stable and efficient genetic transformation is still an important problem in modern biology. It is assumed
Yufei Xia +7 more
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Genetic Transformation in Yeasts [PDF]
SUMMARY: The transformability of 25 strains of yeasts belonging to 23 species in three genera - Saccharomyces, Hansenula and Candida - was investigated with respect to ten characters. Eighteen hour cultures at about the end of the log phase of growth, addition of 10 μg DNA/ml, and an incubation time of 30 min at 30 to 37 °C usually yielded best results.
N C, Khan, S P, Sen
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From “the dialectics of nature” to the inorganic gene [PDF]
The concept of projection from one space to another, with a consequent loss of information, can be seen in the relationships of gene to protein and language description to real situation.
Mackay, Alan L.
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Marker-free transgenic plants through genetically programmed auto-excision [PDF]
We present here a vector system to obtain homozygous marker-free transgenic plants without the need of extra handling and within the same time frame as compared to transformation methods in which the marker is not removed.
Verweire, Dimitri +4 more
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Genetic Transformation in Rhizobium [PDF]
SUMMARY: Mutants of ground nut (Rhizobium cowpea group) strain gr3 were isolated using ultraviolet radiation and N-methyl-N'-nitrosoguanidine (NTG) as mutagenic agents. An adenine-requiring mutant was used as the recipient and its wild type as the donor in transformation studies. Initially minimal medium supplemented with 2 μg. adenine/ml. was used for
J L, Raina, V V, Modi
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Continuous dynamic problem generators for evolutionary algorithms [PDF]
This article is posted here with permission from IEEE - Copyright @ 2007 IEEEAddressing dynamic optimization problems has attracted a growing interest from the evolutionary algorithm community in recent years due to its importance in the applications of ...
Tinos, R, Yang, S
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Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]
The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes +5 more
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Background Eruca vesicaria subsp. sativa is one of the Cruciferae species most tolerant to drought stress. In our previous study some extremely drought-tolerant/sensitive Eruca lines were obtained.
Bang-Lian Huang +7 more
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Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum) [PDF]
To develop an efficient protocol for shoot regeneration of root chicory (Cichorium intybus var. sativum), some factors, including different concentrations of plant growth regulators in Murashige and Skoog (MS) medium, type of explants and genotypes were ...
De Loose, Marc +4 more
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Xylanases belong to an important class of industrial enzymes. Various xylanases have been purified and characterized from a plethora of organisms including bacteria, marine algae, plants, protozoans, insects, snails and crustaceans.
Amit Bhardwaj +5 more
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