Results 201 to 210 of about 1,392,091 (269)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Reflections on Healthcare Leadership in KwaZulu-Natal, South Africa During the COVID-19 Pandemic: Implications for Crisis Leadership Development and Implementation. [PDF]

J Healthc Leadersh
Ramklass SS.
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Correction: Building Health Policy and Systems Research (HPSR) capacity in India: reflections from the India HPSR fellowship program (2020–2023)

Health Research Policy and Systems
Shilpa John, Sudha Ramani, Syed Mohd Abbas, Sumit Kane, Dorothy Lall, Prashanth N. Srinivas, Devaki Nambiar, Bruno Marchal, Sara Van Belle, Rajeev Sadanandan, Narayanan Devadasan   +10 more
doaj   +1 more source

From recalcitrance to precision: a robust regeneration, transformation and targeted gene editing framework in <i>Cajanus cajan</i>. [PDF]

Front Genome Ed
Verma R, Bharti J, Thangaraj A, Sony SK, Gupta I, Chakraborty P, Kaul R, Rawat B, Maithreyi RS, Samantaray JP, Preet S, Tanwar K, Bhardwaj D, Kaul T.   +13 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Cas9/sgRNA-mediated genome editing of citrus via mature tissue transformation enables both high-efficacy genome editing and early flowering. [PDF]

Front Plant Sci
Jia H, Hu Z, Wu H, Duan Y, Zale J, Wang N.   +5 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Effects of long-term tillage on soil nitrogen transformation, nitrogen fractions, and wheat yield. [PDF]

Front Plant Sci
Wang Q, Shi Y, Yu Z, Zhang Y, Zhang Z.
europepmc   +1 more source

Atomic-Scale Mechanisms of Nanoscale Material Removal in FeCrNiCoCu High-Entropy Alloys: Coupled Effects of Crystallography, Grain Size, and Composition. [PDF]

Nanomaterials (Basel)
Ling X, Fu P, Li Y, Zhou Z, Li Z.
europepmc   +1 more source