Results 231 to 240 of about 6,184,664 (298)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

A Once-in-a-Generation Transformation at an Academic Public Hospital During Federal Funding Uncertainty. [PDF]

J Healthc Leadersh
Spellberg B, Coffey CE, Bickley L, Ross W, Kaiser LR, Yee HF.   +5 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

<i>RUBY</i>-Mediated Visual Selection Facilitates Transgenic Screening and Red Floral Pigmentation in <i>Petunia</i> × <i>hybrida</i>. [PDF]

Plants (Basel)
Yao J, Yan F, Chandrasekaran A, Aung TT, Youn S, Kim Y, Lee GJ.   +6 more
europepmc   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Correction for Lim et al., Longitudinal transformation of mitochondrial metabolism during neurogenesis. [PDF]

Proc Natl Acad Sci U S A
europepmc   +1 more source

Developmental regulators enable rapid and efficient soybean transformation and CRISPR-mediated genome editing. [PDF]

Plant Physiol
Alok A, Raman V, D'Agostino L, Kshetry AO, Rai KM, Wang C, Gunapati S, Stupar RM, Patil GB, Zhang F.   +9 more
europepmc   +1 more source

Immune suppression, HLA-risk and potential future immune based targets of oral leukoplakia progression. [PDF]

Front Dent Med
Soni M, DeSantis B, Villa A, Kamoun M, Gates J.   +4 more
europepmc   +1 more source

Chromosome-level genome assembly, annotation, evolutionary analysis, and establishment of transformation systems in Carex rigescens. [PDF]

Plant Cell Rep
Wang L, Ma C, Li Y, Zhu K, Wang C, Wu Y, Hu Q, Sun Y.   +7 more
europepmc   +1 more source