Results 11 to 20 of about 443,920 (343)
Genetic Transformation in Citrus [PDF]
The Scientific World Journal, 2013 Citrus is one of the world’s important fruit crops. Recently, citrus molecular genetics and biotechnology work have been accelerated in the world. Genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable ...
Dicle Donmez +4 more
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Insights into the genetic influences of the microbiota on the life span of a host
Frontiers in Microbiology, 2023 Escherichia coli (E. coli) mutant strains have been reported to extend the life span of Caenorhabditis elegans (C. elegans). However, the specific mechanisms through which the genes and pathways affect aging are not yet clear.
Fang Zhang +11 more
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BMC Genetics, 2020 Background Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear.
Fu-shuang Dong +9 more
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Genetic Transformation of Yeast [PDF]
BioTechniques, 2001 Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S.
R. D. Gietz, Robin A. Woods
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Establishment of a Genetic Transformation System in Guanophilic Fungus Amphichorda guana
Journal of Fungi, 2021 Fungi from unique environments exhibit special physiological characters and plenty of bioactive natural products. However, the recalcitrant genetics or poor transformation efficiencies prevent scientists from systematically studying molecular biological ...
Min Liang +5 more
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Genetic Transformation in Yeasts [PDF]
Journal of General Microbiology, 1974 SUMMARY: The transformability of 25 strains of yeasts belonging to 23 species in three genera - Saccharomyces, Hansenula and Candida - was investigated with respect to ten characters. Eighteen hour cultures at about the end of the log phase of growth, addition of 10 μg DNA/ml, and an incubation time of 30 min at 30 to 37 °C usually yielded best results.
N. C. Khan, S. P. Sen
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Plants, 2022 Sequence and expression data obtained by next-generation sequencing (NGS)-based forward genetics methods often allow the identification of candidate causal genes. To provide true experimental evidence of a gene’s function, reverse genetics techniques are
Mohsen Niazian +2 more
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Genetic Transformation in Rhizobium [PDF]
Journal of General Microbiology, 1969 SUMMARY: Mutants of ground nut (Rhizobium cowpea group) strain gr3 were isolated using ultraviolet radiation and N-methyl-N'-nitrosoguanidine (NTG) as mutagenic agents. An adenine-requiring mutant was used as the recipient and its wild type as the donor in transformation studies. Initially minimal medium supplemented with 2 μg. adenine/ml. was used for
V. V. Modi, J. L. Raina
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Genetic Transformation in Pseudomonas [PDF]
Journal of General Microbiology, 1967 SUMMARY: Intraspecific and interspecific transformation in Pseudomonas has been investigated. Two isoleucine+valine requiring mutants of Pseudomonas aeruginosa have been transformed to prototrophy. By using gelatin liqui-faction as a marker character it was observed that two strains of P. fluorescens, a phytopathogen P.
N. C. Khan, S. P. Sen
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PheNominal: an EHR-integrated web application for structured deep phenotyping at the point of care
BMC Medical Informatics and Decision Making, 2022 Background Clinical phenotype information greatly facilitates genetic diagnostic interpretations pipelines in disease. While post-hoc extraction using natural language processing on unstructured clinical notes continues to improve, there is a need to ...
James M. Havrilla +8 more
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