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Simplified floral dip transformation method of Arabidopsis thaliana.

Journal of Microbiological Methods, 2022
Being a model plant, Arabidopsis transformation is a frequent routine in genetics and molecular laboratories. Here, we explain a modified floral dip method routinely used in our laboratory for Arabidopsis transformation by applying drought stress after ...
Iftikhar Ali   +4 more
semanticscholar   +1 more source

Genetic transformation.

2011
Genetic transformation protocols are now available for all major grain legume crops. Nevertheless the majority of these species remain difficult to transform, mostly because they are recalcitrant for in vitro regeneration. This review aims to discuss the problems and achievements in grain legume regeneration and transformation and the factors affecting
Angenon, Geert, Tran, Thanh Thu
openaire   +2 more sources

Genetic transformation of BCG

Tubercle, 1989
Two substrains of BCG, the Pasteur and Japanese, were successfully transformed with E. coli-mycobacteria shuttle plasmids, constructed from the E. coli plasmid, pIJ666 and the M. fortuitum plasmid, pAL5000. Individual plasmids (pYUB13, pYUB14) were obtained that contain selectable antibiotic resistance markers for kanamycin and chloramphenicol ...
L, Lugosi, W R, Jacobs, B R, Bloom
openaire   +2 more sources

Genetic transformation of fungi

The International Journal of Developmental Biology, 2017
Transferring DNA into cells is an essential research method for molecular cloning and gene function studies. As molecular biology and materials physics develop, more and more new transformation methods have been applied to mammalian cells. Some techniques have been successfully developed for several types of fungi, but their efficiencies are extremely ...
Liya, He   +7 more
openaire   +2 more sources

The population genetics of urban and rural amphibians in North America

Molecular Ecology, 2021
Human land transformation is one of the leading causes of vertebrate population declines. These declines are thought to be partly due to decreased connectivity and habitat loss reducing animal population sizes in disturbed habitats.
C. Schmidt, C. Garroway
semanticscholar   +1 more source

Genetic Transformation of Switchgrass

2009
Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used
Yajun, Xi, Yaxin, Ge, Zeng-Yu, Wang
openaire   +2 more sources

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