Results 41 to 50 of about 2,864,901 (314)

Testicular Germ Cell Tumours: A Review of Genetics, Morphology and Immunohistochemistry [PDF]

open access: yesNational Journal of Laboratory Medicine, 2015
Testicular Germ Cell Tumours (TGCTs) are rare tumours but they are the most common solid malignancy in young males and second leading cause of mortality. They exhibit biological diversity making them interesting for academics.
Kanwardeep Kaur Tiwana   +4 more
doaj   +1 more source

Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]

open access: yes, 2020
The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes   +5 more
core   +1 more source

Genetic Transformation of Yeast [PDF]

open access: yesBioTechniques, 2001
Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S.
R. Daniel Gietz, Robin A. Woods
openaire   +3 more sources

Tomato: a crop species amenable to improvement by cellular and molecular methods [PDF]

open access: yes, 1989
Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures.
A. Crossway   +192 more
core   +3 more sources

A one locus, biased mutation model and its equivalence to an unbiased model [PDF]

open access: yes, 2004
Experimental data suggests that for some continuously varying characters under stabilising selection, mutation may cause a mean change in the value of the character. A one locus, mathematical model of a continuously varying biological character with this
Abramowitz   +16 more
core   +2 more sources

Genetic Transformation in Rhizobium [PDF]

open access: yesJournal of General Microbiology, 1969
SUMMARY: Mutants of ground nut (Rhizobium cowpea group) strain gr3 were isolated using ultraviolet radiation and N-methyl-N'-nitrosoguanidine (NTG) as mutagenic agents. An adenine-requiring mutant was used as the recipient and its wild type as the donor in transformation studies. Initially minimal medium supplemented with 2 μg. adenine/ml. was used for
J L, Raina, V V, Modi
openaire   +2 more sources

A CRISPR-Cas9 sex-ratio distortion system for genetic control. [PDF]

open access: yes, 2016
Genetic control aims to reduce the ability of insect pest populations to cause harm via the release of modified insects. One strategy is to bias the reproductive sex ratio towards males so that a population decreases in size or is eliminated altogether ...
Bernardini, F   +9 more
core   +3 more sources

Characterization of serine proteinase expression in agaricus bisporus and coprinopsis cinerea by using green fluorescent protein and the A. bisporus SPR1 Promoter [PDF]

open access: yes, 2008
The Agaricus bisporus serine proteinase 1 (SPR1) appears to be significant in both mycelial nutrition and senescence of the fruiting body.
Andy M. Bailey   +13 more
core   +4 more sources

Highly efficient transformation of the model zygnematophycean alga Closterium peracerosum-strigosum-littorale complex by square-pulse electroporation.

open access: yesNew Phytologist, 2021
The zygnematophycean algae occupy an important phylogenetic position as the closest living relatives of land plants. Reverse genetics is quite useful for dissecting the functions of genes. However, this strategy requires genetic transformation, and there
J. Kawai   +5 more
semanticscholar   +1 more source

One-Step Assembly of a Porcine Epidemic Diarrhea Virus Infectious cDNA Clone by Homologous Recombination in Yeast: Rapid Manipulation of Viral Genome With CRISPR/Cas9 Gene-Editing Technology

open access: yesFrontiers in Microbiology, 2022
Porcine epidemic diarrhea virus (PEDV), a swine enteric coronavirus causing acute diarrhea in piglets, is one of the major threatens to the pork industry globally.
Yanyang Zhou   +10 more
doaj   +1 more source

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