Results 41 to 50 of about 171,516 (260)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Preparation and evaluation of Brucella T4SS recombinant proteins in serodiagnosis of human brucellosis based on TMT-based proteomics technology

Frontiers in Cellular and Infection Microbiology
IntroductionBrucellosis, a significant zoonotic infectious disease, poses a global health threat. Accurate and efficient diagnosis is crucial for prevention, control, and treatment of brucellosis. VirB proteins, components of the Type IV secretion system
Qi Wu, Chen Sun, Liping Guo, Yujia Xie, Jinpeng Zhang, Jinpeng Zhang, Dehui Yin, Dehui Yin   +7 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging

Frontiers in Nutrition, 2023
The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation.
Xupeng Xing, Xupeng Xing, Jingfeng Peng, Jingfeng Peng, Jingyu Zhao, Jingyu Zhao, Ruoxi Shi, Ruoxi Shi, Caiqin Wang, Caiqin Wang, Zihan Zhang, Zihan Zhang, Zihan Wang, Zihan Wang, Zicong Li, Zicong Li, Zhenfang Wu, Zhenfang Wu   +17 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Preparation and application of specific chicken yolk antibodies in detecting Brucella

Frontiers in Veterinary Science
Brucellosis, caused by Brucella, is a severe zoonotic disease. Conventional IgG antibody-based ELISA testing faces challenges such as false positives and cross-reactivity.
Xinru Qi, Shiqi Zhao, Qianhan Huang, Yun Wang, Qichuan Pei, Yixiao Chen, Dehui Yin, Dehui Yin, Tiansong Zhan, Tiansong Zhan   +9 more
doaj   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

Rapid generation of HCoV-229E and HCoV-OC43 reporter viruses and replicons for antiviral research

Frontiers in Cellular and Infection Microbiology
IntroductionThe large size of coronavirus genome, along with the instability of certain genomic sequences, makes the construction of reverse genetics for coronaviruses particularly challenging.
Yuyuan Zhang, Jiannan Chen, Hui Feng, Lulu Yang, Liyuan Hu, Yang Gao, Ziqiao Wang, Fei Feng, Jincun Zhao, Ping Zhang, Rong Zhang   +10 more
doaj   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Integration of circadian and hypoxia signaling via non‐canonical heterodimerization

FEBS Letters, EarlyView.
CLOCK, BMAL1, and HIFs are basic helix‐loop‐helix and Per‐Arnt‐Sim domain (bHLH‐PAS) proteins, which function as transcription factors. bHLH‐PAS proteins are designated in two classes. Many class I proteins are regulated by environmental signals via their PAS domains, but such signals have not been identified for all.
Sicong Wang, Katja A. Lamia
wiley   +1 more source