Results 121 to 130 of about 21,720 (312)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Features of Traction Transformer Windings Utilised as Inductances

Advances in Electrical and Electronic Engineering, 2004
The paper analyses features of traction transformer desing. Secondary windings of the transformer are utilized as a choke of an locomotive input filter at DC supplying system.
Jiri Danzer
doaj  

Numerická analýza a simulace Rogowského cívky [PDF]

, 2019
This work illustrates an analysis of Rogowski coils for power applications, when operating under non ideal measurement conditions. The developed numerical model, validated by comparison with other methods and experiments, enables to investigate the ...
Hussion, M Walid
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Improved Measurement Method for Locating Winding Faults in On-Board Transformers in High-Speed Trains

IEEE Access
Frequency response analysis (FRA) is a widely used fault diagnosis approach for transformer windings. However, its application to on-board transformers in high-speed trains faces challenges because the four groups of high-voltage (HV) windings are often ...
Zhenyu Wu, Tao Wang, Juan Yan, Kang Li, Hui Wang, Cungang Hu   +5 more
doaj   +1 more source

Carbon transforms wind farm access

Reinforced Plastics, 2015
A new form of marine craft, one minute a veritable greyhound of the seas and the next a steady platform for embarking from, has arisen over the last few years for serving offshore wind farms. Advanced low-weight composites are key enabler for these wind farm service vessels (WFSVs).
openaire   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Application of saturated core transformer as inrush current limiter

e-Prime: Advances in Electrical Engineering, Electronics and Energy
An inrush current is a transient current that results from a sudden change in the exciting voltage across a transformer's windings. Reducing the inrush current of transformers is desirable due to mechanical stresses and negligent operation of protective ...
Mehrdad Tarafdar-Hagh, Noorallah Hasanlouei, Kashem Muttaqi, Kamran Taghizad-Tavana, Sayyad Nojavan, Meysam Abdi   +5 more
doaj   +1 more source