Results 71 to 80 of about 1,469 (267)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

Short Circuit Stress Analysis Using FEM in Power Transformer on H-V Winding Displaced Vertically & Horizontally

open access: yesAlexandria Engineering Journal, 2018
The aim of this work was to work out the mechanical stresses within transformer resulting from the extreme short-circuit currents. The forces and stresses set up in transformer windings as the result of exterior or interior short-circuits or of switching
Ashfaq Ahmad   +3 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

The Application of Transformers with High-Temperature Superconducting Windings Considering the Skin Effect in Mobile Power Supply Systems

open access: yesMathematics
The active and passive components of transformer electrical equipment have reached their limits regarding modernization and optimization, leading to the implementation of innovative approaches.
Vadim Manusov   +4 more
doaj   +1 more source

Diagnosis of axial displacement in transformer windings using finite element analysis

open access: yesJournal of Mathematical Sciences and Modelling, 2018
Transformer is one of the main equipment in the power system and power quality of the power system mainly depends on the working of the power transformer. Leakage reactance is one of the main parameters of the transformer.
Kamran Dawood   +2 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

A Novel Transformer-Based Single-to-Three Phase Conversion Technique Using Rotating Magnetic Field Theory

open access: yesIranian Journal of Electrical and Electronic Engineering, 2021
This paper presents a new single to three phase converter using rotating magnetic field transformer. Conventional transformers have been used in many converters aiming at supplementary improvements and they usually have no critical effect on the ...
S. R. Mousavi-Aghdam, N. Elahi Kachaei
doaj  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

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