Results 311 to 320 of about 361,542 (380)

Substantial enhancement of <i>Agrobacterium</i>-mediated transgene-free genome editing via short-term chemical selection using citrus as a model plant. [PDF]

Hortic Res
Li Y, Liu Z, Gmitter FG, Deng Z, Cheng B, Duan H, Li Y.   +6 more
europepmc   +1 more source

New biosensors and transgenic mice for multiplex cGMP imaging

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a versatile second messenger that is important for human (patho‐)physiology and pharmacotherapy. Live‐cell imaging of cGMP with biosensors allows to elucidate its spatiotemporal dynamics in real time under close‐to‐native conditions. However, to monitor two separate cGMP pools or cGMP/cAMP
Markus Wolters, Martin Thunemann, Barbara Birk, Susanne Feil, Viacheslav O. Nikolaev, Moritz Lehners, Robert Feil   +6 more
wiley   +1 more source

Generation and characterization of a humanized <i>ACE2</i> rat model for the study of SARS-CoV-2 and COVID-19. [PDF]

Front Microbiol
Olson RM, Nabli H, Gentry BA, Davis DJ, Franklin CL, Shaw ML, Bryda EC.   +6 more
europepmc   +1 more source

Unraveling the Genetic Mysteries of Müllerian Anomalies: Research Approaches and Clinical Significance

Clinical Genetics, EarlyView.
This review primarily summarizes the genetic defects in Müllerian anomalies, the tools used to validate these genetic defects, and the future clinical significance of identifying the precise genetic etiology of Müllerian anomalies. ABSTRACT Müllerian anomalies are a collection of heterogeneous anatomical disorders of the female genital tract that ...
Jingfang Li, Xin Hou, Xiangyu Wang, Juan Li, Li Li, Xiangyi Ma   +5 more
wiley   +1 more source

A rapid and dynamic role for FMRP in the plasticity of adult neurons. [PDF]

Nat Commun
Gundermann DG, Lymer S, Lennon J, Blau J.   +3 more
europepmc   +1 more source

Identification and Phylogenetic Characterisation of Novel Adeno‐Associated Virus Capsids in Non‐Human Primate Tissues

Cell Proliferation, EarlyView.
AAV's widespread application is currently confronting critical challenges. This study established a systematic framework of ‘natural discovery–evolutionary analysis–functional optimization’, providing a new paradigm for the development of next‐generation AAV vectors with clinical‐grade tissue specificity, low immunogenicity, and cross‐species ...
Liyu Zhu, Kai Xu, Yali Ding, Kailun Liu, Jing Liu, Zongren Hou, Rui Niu, Ning Yang, Hualing Qin, Baoyang Hu, Ying Zhang, Wei Li   +11 more
wiley   +1 more source

A temperature-sensitive CRISPR-Cas12a system for sterile insect technique. [PDF]

Nat Commun
Nguyen C, Omotayo AI, Sanz Juste S, Feng X, López Del Amo V.   +4 more
europepmc   +1 more source

Stage‐Associated Microglial Subpopulations and Dynamics in Vascular Pathogenesis of Oxygen‐Induced Retinopathy

Cell Proliferation, EarlyView.
In the oxygen‐induced retinopathy (OIR) mouse model, two distinct microglial subpopulations play opposing roles: highly glycolytic microglia (HGM) promote neovascular formation via Pkm2, while Mrc1/CD206‐positive phagocytosis‐associated microglia (PAM) facilitate its regression.
Yuan Ma, Ziye Chen, Baoyi Liu, Wen Ding, Runping Duan, Kangjie Kong, Zhuojun Xu, Jizhu Li, Jiali Ru, Dianlei Guo, Xiaoyue Wei, Yaping Liu, Zhuangling Lin, Yang Meng, Yuan Liu, Lan Jiang, Zitong Chen, Rebiya Tuxun, Chinling Tsai, Chunqiao Liu, Tao Li   +20 more
wiley   +1 more source

Preventing secondary primary malignancies (SPMs) in CAR-T cell therapy through site-specific transgene integration into genomic safe harbors (GSHs). [PDF]

J Transl Med
Safarzadeh Kozani P, Safarzadeh Kozani P.   +1 more
europepmc   +1 more source

Correlation Between Chimeric Antigen Receptor T-Cell Pharmacokinetic Data Measured by Flow Cytometry and Quantitative Polymerase Chain Reaction. [PDF]

Clin Transl Sci
Wang W, Liu X, Yang T, Wang J, Liu J, Wang Y, Yu J, Luo T, Wang X, Li J, Wang C, Chen N, Geng D, Xu D.   +13 more
europepmc   +1 more source