Results 181 to 190 of about 70,580 (269)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Uncovering NMDA receptor-mediated high firing activity in excitatory human neurons. [PDF]

iScience
Gameiro-Ros I, Tengolics AJ, Prytkova I, Kamarajan C, Pang ZP, Goate AM, Hart RP, Slesinger PA.   +7 more
europepmc   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Functional synaptic connectivity shapes spine stability in the hippocampus. [PDF]

Nat Commun
Rais C, Wiegert JS.
europepmc   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Time-resolved spectroscopy of a photoactive dinuclear W/Ru complex: spectroscopic evidence for a metastable intermediate with side-on coordinated carbonyl ligand. [PDF]

Chem Sci
Borter JH, Kangsa Banik S, Kunze K, Kupfer S, Schwarzer D, Seidel WW.   +5 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Susceptibility to Pitting Corrosion of AerMet 100 and 4340 Alloys for Aeronautical Applications. [PDF]

Materials (Basel)
Huerta-Zavala MS, Gaona-Tiburcio C, Nieves-Mendoza D, Jaquez-Muñoz JM, Cabral-Miramontes J, Maldonado-Bandala E, Croche-Belin R, Baltazar-Zamora MA, Landa-Ruiz L, Lopez-Leon LD, Olguin-Coca J, Almeraya-Calderon F.   +11 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Slow slip modulates low-frequency seismicity on the Parkfield segment of the San Andreas Fault. [PDF]

Nat Commun
Zali Z, Martínez-Garzón P, Mencin D, Beroza GC.   +3 more
europepmc   +1 more source