Results 31 to 40 of about 15,169 (268)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Research on Shanghai Rail Transit Signaling System Evolution Scheme

Chengshi guidao jiaotong yanjiu
Objective With the rapid development of urban rail transit, the operational volume of trains has significantly increased. The existing CBTC (communication-based train control) system can no longer meet the signaling system needs of high-density ...
WANG Lizhou
doaj   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Integrated Development of Urban and Regional Rail Transit Networks [PDF]

中国工程科学
As China's urbanization accelerates, single-mode rail transit systems are no longer sufficient to satisfy increasingly diverse commuting needs. There is a growing demand for an integrated, multi-level rail transit model tailored to China's specific ...
Rui Wang, Qigang Liu, Xiao Yang, Kun Qian, Yuan Jiang, Yanan Zhang, Jingjing Bao   +6 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Strategy for Selecting Signaling System Types for Suburban Railways

Chengshi guidao jiaotong yanjiu
ObjectiveThe mainstream signaling systems for suburban railways are CTCS2+ATO （Chinese train control system level 2 + automatic train operation） and CBTC （communication-based train control）.
Lin CHEN, Rui SONG, Ning DANG, Yifan QIU
doaj   +1 more source

Cybersecurity Situational Awareness System Applicable for Urban Rail Transit CBTC System

Chengshi guidao jiaotong yanjiu
Objective Urban rail transit CBTC (communication-based train control) systems face complex and diverse cybersecurity challenges. Existing cybersecurity tools feature high false alarm rates and poor adaptation to CBTC systems, lacking in-depth analysis of
LIU Dongdong, ZHOU Xingyu, DUO Jianhua, WANG Xiangyang, HAN Tao, ZHU Suoming   +5 more
doaj   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

The Research on Delay Propagation of Urban Rail Transit Operation under Sudden Failure

Journal of Advanced Transportation, 2021
With the increase and extension of urban rail transit lines, networked operation has become an inevitable trend of rail transit operations. Once an emergency occurs, it will cause operational delays; in serious cases, it may further lead to group safety ...
Yuan Zhao, Xiaobing Ding
doaj   +1 more source

Integrating COVID-19 health risks into crowding costs for transit schedule planning

Transportation Research Interdisciplinary Perspectives, 2022
The public transport sector worldwide experienced the worst impact in recent history, in terms of ridership loss, due to the COVID-19 pandemic. The pandemic negatively affected passengers' perceptions of public transport and is likely to make a lasting ...
K.W. Devasurendra, Saeid Saidi, S.C. Wirasinghe, Lina Kattan   +3 more
doaj   +1 more source