Results 181 to 190 of about 1,545,528 (302)

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Computational prediction-combined proteogenomics unveils widespread non-AUG translation initiation events in plants. [PDF]

Nucleic Acids Res
Zhang Y, Chang B, Wang S, Tian L, Zhao H, Luo W, Zhang H, Zhang S, Wu S, Wu L.   +9 more
europepmc   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

The mechanism of ribosomal recruitment during translation initiation on the Type 2 encephalomyocarditis virus IRES. [PDF]

EMBO J
Bhattacharjee S, Abaeva IS, Brown ZP, Arhab Y, Fallah H, Hellen CUT, Frank J, Pestova TV.   +7 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

TRAINSPOTTER: profiling nascent protein N-termini indicative of bacterial translation initiation via deformylation-assisted N-terminomics. [PDF]

Nucleic Acids Res
Van Damme P.
europepmc   +1 more source

Human AGO1 5’UTR mediates an elF4G-enhanced but cap-independent mechanism of translation initiation

Argonaute 1 (AGO1) is an essential effector in RNA-mediated gene silencing pathways. It regulates developmental control and stem cell maintenance, and is related to tumourigenesis. Such functions suggest its expression must be tightly regulated and, most
Lacerda, Rafaela, Romão, Luísa, Menezes, Juliane   +2 more
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Synergistic targeting of eIF4A-mediated translation initiation and apoptosis in acute myeloid leukemia. [PDF]

Blood Neoplasia
Lee YS, Good JD, Awais N, Eschle BK, Lineberry MS, Lin J, Wunderlich M, Thibodeau J, Edwards H, Teo JL, Bibeau S, Wollman M, Karadimov G, Sariipek N, Salik B, Acosta J, Noel J, Cucchi DGJ, DePasquale EAK, Gokhale PC, Ritz J, Ge Y, Miller PG, Najm FJ, Stone RM, Garcia JS, Lane AA, Jones CL, van Galen P.   +28 more
europepmc   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source