Results 261 to 270 of about 314,589 (303)

lacZ translation initiation mutations

Journal of Molecular Biology, 1984
Sixteen single point mutations near the beginning of the lacZ gene have been isolated and their effect on lacZ expression has been measured. Five mutations were obtained that alter a potential stem-and-loop structure in the messenger RNA that masks the initiation codons.
L M, Munson, G D, Stormo, R L, Niece, W S, Reznikoff   +3 more
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Reconstitution of Yeast Translation Initiation

2007
To facilitate the mechanistic dissection of eukaryotic translation initiation we have reconstituted the steps of this process using purified Saccharomyces cerevisiae components. This system provides a bridge between biochemical studies in vitro and powerful yeast genetic techniques, and complements existing reconstituted mammalian translation systems ...
Michael G, Acker, Sarah E, Kolitz, Sarah F, Mitchell, Jagpreet S, Nanda, Jon R, Lorsch   +4 more
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Regulation of translation initiation by herpesviruses

Biochemical Society Transactions, 2008
Viruses are dependent upon the host cell protein synthesis machinery, thus they have developed a range of strategies to manipulate host translation to favour viral protein synthesis. Consequently, the study of viral translation has been a powerful tool for illuminating many aspects of cellular translational control.
Richard W P, Smith, Sheila V, Graham, Nicola K, Gray   +2 more
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Translation initiation and viral tricks

Trends in Biochemical Sciences, 2003
A variety of viral strategies are utilized for dominance of the host-cell protein synthetic machinery, optimization of viral mRNA translation and evasion of host-cell antiviral responses that act at the translational level. Many viruses exploit regulated steps in the initiation of cellular protein synthesis to their own advantage.
Robert J, Schneider, Ian, Mohr
openaire   +2 more sources

Translation initiation in colorectal cancer

Cancer and Metastasis Reviews, 2012
Colorectal cancers (CRC) are one of the most common causes of morbidity and mortality in high-income countries. Targeted screening programs have resulted in early treatment and a substantial decrease in mortality. However, treatment strategies for CRC still require improvement.
Armen, Parsyan, Greco, Hernández, Sarkis, Meterissian   +2 more
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A hypothesis on the mechanism of translational initiation

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1998
[No abstract available]
openaire   +3 more sources

Initiation of Translation

1999
The vacant ribosomal particles after termination may be again involved in translation. Several mechanisms of initiation of translation of a new message, or reinitiation of translation of the next coding sequence of the same polycistronic mRNA (in prokaryotes), or reinitiation of translation of the same monocistronic mRNA (in eukaryotes) by vacant post ...
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Translation initiation: adept at adapting

Trends in Biochemical Sciences, 1999
Initiation of protein synthesis requires both an mRNA and the initiator methionyl (Met)-tRNA to be bound to the ribosome. Most mRNAs are recruited to the ribosome through recognition of the 5' m7G cap by a group of proteins referred to as the cap-binding complex or eIF4F.
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In Vitro Studies of Archaeal Translational Initiation

2007
Initiation is the step of translation that has incurred the greatest evolutionary divergence. In silico and experimental studies have shown that archaeal translation initiation resembles neither the bacterial nor the eukaryotic paradigm, but shares features with both.
BENELLI, DARIO, LONDEI, Paola
openaire   +3 more sources

Analysis and reconstitution of translation initiation in vitro

2002
Publisher Summary This chapter discusses the analysis and reconstitution of translation initiation in vitro . Translation initiation is the rate-limiting step in protein biosynthesis, and alteration of the initiation factors by covalent modification—such as phosphorylation—or by mutation can have dramatic effects on the rate of protein synthesis ...
Asano, Katsura, Phan, Lon, Krishnamoorthy, Thanuja, Pavitt, Graham D.; id_orcid 0000-0002-8593-2418, Gomez, Edith, Hannig, Ernest M., Nika, Joseph, Donahue, Thomas F., Huang, Han Kuei, Hinnebusch, Alan G.   +9 more
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