Results 121 to 130 of about 5,287,692 (251)
This paper examines the disparity between the perceptions of private and public transport from those who might exercise choice between them. There is a great body of literature concerning the identification of problems in public transport mobility.
Coxon, Selby +2 more
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We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
CURRENT CHALLENGES IN THE ECONOMICS OF TRANSPORT SYSTEMS – a stakeholder and club good approach [PDF]
In this article, after discussing the concept of transport systems and the importance of its sustainability, stakeholder theory and club good theory are presented as useful instruments for analyzing different challenges for the sustainability of ...
Platje, Joost; International University of Logistics and Transport
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Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Metropolitan passenger transport routes [cartographic material] /
Includes inserts of Perth city block and Spearwood-Jandapot-Forrestdale-Armadale area.; Also available in an electronic version via the internet at: http://nla.gov.au/nla.map-vn4781383.
Western Australia. Transport Board
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Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Multimodal Freight Transport Planning: A Toolbox Supporting Stakeholders’ Collaboration
This paper aims to analyse the planning of chemical transports’ shift from road to the more complex multimodal freight transport, and presents a toolbox developed to support the stakeholders in the process.
Cichosz, Marzenna; SGH Warsaw School of Economics, Department of Transport
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The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
New Zealand transport domain plan [PDF]
The Transport Domain Plan (the Domain Plan) ensures that the transport sector has the right data and information. It aims to help the transport sector to better coordinate how agencies collect and manage data and knowledge, and ensure that existing ...
Ministry of Transport (New Zealand)
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