Results 121 to 130 of about 5,287,692 (251)

The role of industrial design in addressing the disparity between user perceptions of public and private transport

open access: yes, 2007
This paper examines the disparity between the perceptions of private and public transport from those who might exercise choice between them. There is a great body of literature concerning the identification of problems in public transport mobility.
Coxon, Selby   +2 more
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

CURRENT CHALLENGES IN THE ECONOMICS OF TRANSPORT SYSTEMS – a stakeholder and club good approach [PDF]

open access: yes, 2012
In this article, after discussing the concept of transport systems and the importance of its sustainability, stakeholder theory and club good theory are presented as useful instruments for analyzing different challenges for the sustainability of ...
Platje, Joost; International University of Logistics and Transport
core  

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

open access: yesFEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima   +7 more
wiley   +1 more source

Metropolitan passenger transport routes [cartographic material] /

open access: yes, 1939
Includes inserts of Perth city block and Spearwood-Jandapot-Forrestdale-Armadale area.; Also available in an electronic version via the internet at: http://nla.gov.au/nla.map-vn4781383.
Western Australia. Transport Board
core  

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Multimodal Freight Transport Planning: A Toolbox Supporting Stakeholders’ Collaboration

open access: yes, 2019
This paper aims to analyse the planning of chemical transports’ shift from road to the more complex multimodal freight transport, and presents a toolbox developed to support the stakeholders in the process.
Cichosz, Marzenna; SGH Warsaw School of Economics, Department of Transport
core  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

open access: yesFEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti   +5 more
wiley   +1 more source

New Zealand transport domain plan [PDF]

open access: yes, 2016
The Transport Domain Plan (the Domain Plan) ensures that the transport sector has the right data and information. It aims to help the transport sector to better coordinate how agencies collect and manage data and knowledge, and ensure that existing ...
Ministry of Transport (New Zealand)
core  

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