Results 71 to 80 of about 101,571 (211)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Dual-mode varifocal Moiré metalens for quantitative phase and edge-enhanced imaging

Nanophotonics
Transport-of-intensity equation (TIE) as a noninterference method for quantitative phase imaging (QPI) has broad applications in micrographic imaging and optical metrology.
Lian Yi, Liu Yongqi, Cheng Dewen, Chi Cheng, Bao Yanjun, Wang Yongtian   +5 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Mass Transport Analysis in an Annular Microchannel Driven by a Time-Periodic Oscillatory Electroosmotic Flow for a Maxwell Fluid Under High Zeta Potential

Advances in Mathematical Physics
This study investigates characteristics of the mass transport for Maxwell fluids driven by a time-periodic oscillatory electroosmotic flow (EOF) in annular microchannels under high zeta potential conditions.
Yuran Qiao, Xiaogang Chen, Jifeng Cui, Xiaonan Zang, Huaizhen Wang   +4 more
doaj   +1 more source

Local correlation-based transition model for aerodynamic flows in wide speed range

Engineering Applications of Computational Fluid Mechanics
A local correlation-based transition model is developed for aerodynamic flows in a wide speed range. New localized methods for the momentum-thickness Reynolds number are introduced for constructing local transition indicators. New transition correlations
Hexia Huang, Zaijie Liu, Huijun Tan
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

MODELING OF ROUGH WALL BOUNDARY LAYERS WITH AN INTERMITTENCY TRANSPORT MODEL

TASK Quarterly, 2010
The paper presents a new extension of the γ-Reθt model to account for both the laminar-turbulent transition and the surface roughness. The new modeling approach takes into account the pressure gradient, turbulence intensity and roughness height and ...
WITOLD ELSNER, PIOTR WARZECHA
doaj  

Simulation of the 2010 September 9 Solar Energetic Particle Event by Using a Data-constrained Transport Model

The Astrophysical Journal
Solar energetic particles (SEPs) can cause damage to spacecraft systems and affect human activities in space. On 2010 September 9, a gradual SEP event was observed by spacecraft STEREO-A, Solar and Heliospheric Observatory (SOHO), and STEREO-B at widely ...
Xinyi Tao, Fang Shen, Xueshang Feng, Wanyi Lian, Bofeng Tang, Yi Yang   +5 more
doaj   +1 more source