Results 81 to 90 of about 1,885,759 (302)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Citizens’ exposure to predominant noise sources in agglomerations

Noise Mapping
Environmental noise directive requires Member States to produce and periodically update strategic noise maps for agglomerates in order to evaluate citizens’ noise exposure before the action plan.
Bolognese Matteo, Fredianelli Luca, Stasi Gianmarco, Ascari Elena, Crifaci Giulia, Licitra Gaetano   +5 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Annual Report for Calendar Year 2017: New England Transportation Consortium [PDF]

The New England Transportation Consortium (NETC) is a cooperative effort of the transportation agencies of the six New England States, the six New England state land grant universities, and the Federal Highway Administration (FHWA).
New England Transportation Consortium
core  

Nonlinear quantum transport and current noise

Annalen der Physik, 2000
Abstract We study nonequilibrium transport and noise in a genericdissipative tightbinding model. Within a real‐time path integral approach, we derive formally exact series expressions in the number of tunneling events for the noise valid for arbitrary bias, frequency and temperature.
Lang, Gunther, Weiss, Ulrich
openaire   +3 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

A survey on exposure-response relationships for road, rail, and aircraft noise annoyance: Differences between continuous and intermittent noise

Environment International, 2019
The aim of the present study is to establish exposure-response relationships reflecting the percentage highly annoyed (%HA) as functions of road traffic, railway, and aircraft noise exposure, measured as day-evening-night level (Lden), as well as to ...
Mark Brink, Beat Schäffer, Danielle Vienneau, Maria Foraster, Reto Pieren, Ikenna C. Eze, Christian Cajochen, Nicole Probst-Hensch, Martin Röösli, Jean-Marc Wunderli   +9 more
doaj   +1 more source

Annual Report for Calendar Year 2016: New England Transportation Consortium [PDF]

The New England Transportation Consortium (NETC) is a cooperative effort of the transportation agencies of the six New England States, the six New England state land grant universities, and the Federal Highway Administration (FHWA).
New England Transportation Consortium
core  

Transportation Expressions

This report compiles definitions of transportation terms used throughout the Department of Transportation and other US government agencies. This is the first edition of Transportation Expressions; future editions will be expanded in scope to include ...
O'Leary, Pamela, Corey, Jay, John A. Volpe National Transportation Systems Center (U.S.)   +2 more
core   +1 more source