Results 71 to 80 of about 44,546 (307)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Journal of Function Spaces, 2022 Aiming at vehicles will produce shocks and vibrations for various reasons in the process of transportation and handling, any shock and vibration that exceeds the regulations would cause damage to the nuclear fuel being transported.
Han Yong +6 more
doaj +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Aircraft Noise Characteristics and Metrics: A PARTNER Project 24 Report [PDF]
Day-Night Average A-weighted Sound Pressure Level (DNL) is used currently to define noise contours around airports and the 65 DNL contour is used as a criterion to determine qualification for noise insulation programs.
Massachusetts Institute of Technology +3 more
core
Noise landing charges and passengers' choice of airport. [PDF]
, 1995 This study demonstrates how aircraft noise can be translated into a form of landing charge. The objectives of the thesis were to develop noise landi'ng charges for six of the major airports in England and to determine what the implication it has on ...
Yeahiya, Mohammed
core
Indian Journal of Public Health, 2014 Background: It is hypothesized that exposure to transportation noise is associated with an increased risk of cardiovascular disease among adult population. The present study further explores this association in the light of new findings. The objective of
Dibyendu Banerjee
doaj +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Citizens’ exposure to predominant noise sources in agglomerations
Noise MappingEnvironmental noise directive requires Member States to produce and periodically update strategic noise maps for agglomerates in order to evaluate citizens’ noise exposure before the action plan.
Bolognese Matteo +5 more
doaj +1 more source